Detection of Genetic markers associated with Plasmodium falciparum drug resistance among malaria patients in Kaduna State, Nigeria
https://dx.doi.org/10.4314/njpar.v42i2.3
Keywords:
Antimalarial, resistance, genes, ampliconsAbstract
Malaria is a disease of public health concern in Nigeria and sub-Saharan Africa. It is caused by intracellular parasites of the genus Plasmodium. The aim of this study was to detect genetic markers associated with Plasmodium falciparum drug resistance among malaria patients in Kaduna State, Nigeria. The study was a cross-sectional study that lasted from May 2018 to October 2018. Three hundred blood samples were collected from consenting individuals attending selected hospitals, in the three senatorial districts of Kaduna State, Nigeria. Structured questionnaire were used to obtain relevant data from study participants. The blood samples were screened for malaria parasites using microscopy and rapid diagnostic test kit. Polymerase Chain Reaction was used for detection of the drug resistance genes. Pfcrt, pfmdr1, pfdhfr, pfdhps and pfatpase6 genes were detected at expected amplicon sizes from the malaria positive samples. The pfatpase6 PCR amplicons were sequenced and a phylogenetic tree was created to determine their relatedness. Result showed that Pfcrt (80%) had the highest prevalence, followed by pfdhfr (60%), pfmdr1 (36%) and pfdhps (8%). Pfatpase6 was also detected in 73.3% of the samples, and a phylogenetic tree showed relatedness between the pfatpase6 sequences in this study and those deposited in the GenBank. In conclusion, the study detected that Plasmodium falciparum genes were associated with drug resistance to commonly used antimalarials.
References
Kebede S,AseffaA, Medhin G, Berhe N, and Velavan TP (2014). Re-evaluation of microscopy confirmed Plasmodium falciparum and Plasmodium vivax malaria by nested PCR detection in southern Ethiopia. Malaria Journal, 13: 48.
Nyarango PM, Gebremeskel T, Mebrahtu G, Mufunda J, Abdulmumini U, Ogbamariam A, Kosia A, Gebremichael A, Gunawardena D, Ghebrat Y, and Okbaldet Y. (2006). A steep decline of malaria morbidity and mortality trends in Eritrea between 2000 and 2004: the effect of combination of control methods. Malaria Journal, 5:333
Ouji M, Augerea J.M, Paloque L and BenoitVical F (2018). Plasmodium falciparum resistance to artemisinin- based combination therapies: A sword of Damocles in the path toward malaria elimination. Parasite, 25:24
WHO (2019). World malaria report 2 0 1 9 . R e t r i e v e d f r o m http://www.who.int/news- room/featurestories/detail/world-malaria-report-2019
World Health Organization (2018) Malaria. Retrieved from https://www.who.int/newsroom/fact-sheets /detail /malaria.
Centers for Disease Control and Prevention (CDC) (2018). Malaria: Drug resistance. R e t r i e v e d f r o m https://www.cdc.gov/malaria/malariaworldwide/reeduction/drug_resistance.html
White NJ (2004). Antimalarial drug r e s i s t a n c e . J o u r n a l o f C l i n i c a l Investigation,113: 1084–1092.
Berzosa P, Esteban-Cantos A, García L, González V, Navarro M, Fernández T, Romay-Barja M, Herrador Z, Rubio JM, Ncogo P, Santana-Morales M, Valladares B, Riloha M &Benito A. (2017). Profile of molecular mutations in pfdhfr, pfdhps, pfmdr1, and pfcrt genes of Plasmodium falciparum related to resistance to different anti- malarial drugs in the Bata District (Equatorial Guinea). Malaria Journal, 16: 28.
Martin, R.E., Ginsburg, H., and Kirk, K. (2009). Membrane transport proteins of the malaria parasite. Molecular Microbiology, 74 (3): 519-28.
Sanchez, C.P., Mayer, S., Nurhasanah, A., Stein, J.W.D and Lanzer, M. (2011). Genetic linkage analyses redefine the roles of Pfcrt and pfmdr1 in drug accumulation and susceptibility in Plasmodium falciparum. Molecular Microbiology, 82(4): 865-78
Sharma, Y. D. (2005). “Genetic alteration in drug resistance markers of Plasmodium falciparum.” Indian Journal of Medical Research, 121(1): 13-22.
Dahlström S (2009). Role of PfATPase6 and pfMRP1 in Plasmodium falciparum resistance to antimalarial drugs. Unit of Infectious Diseases, Department of Medicine, Karolinska University Hospital, Karolinska Institutet, Stockholm, Sweden. Published by Karolinska Institutet. Printed by E-print. ISBN 978-91-7409-481-7.
Aliyu MM, Nasir IA, Umara YA, Vanstawaa AP, Meduguc JT, Emeribed AU, and Amadue DO (2017). Prevalence, risk factors, and antimalarial resistance patterns of falciparum plasmodiasis among pregnant women in Kaduna metropolis, Nigeria. Tzu Chi Medical Journal, 29 (2): 98-103.
Kaltungo, B.Y., Saidu, S.N.A., Sackey, A.K.B. and Kazeem, H.M. (2013). Serological evidence of Brucellosis in Goats in Kaduna North Senatorial District of Kaduna State, Nigeria. International Scholarly Research Notices Veterinary Science,3. DOI: 10.1155/2013/963673.
Naing L, Winn T, and Rusli BN (2006). Practical issues in calculating the sample size for prevalence studies. Archives of Orofacial Sciences, 1: 9-14.
Cheesebrough M (2009). District laboratory practice in tropical countries part 1, second edition. New york: Cambridge University Press.pp. 245-249
Zhang GQ, Guan YY, Sheng HH, Zheng B, Wu S, Xiao, HS & Tang LH (2008). Multiplex PCR and oligonucleotide microarray for detection of singlenucleotide polymorphisms associated with Plasmodium falciparum drug resistance. Journal of Clinical Microbiology, 46 (7), 2167-2174.
Mayenge PI, Ndounga M, Davvy MM, Tandou, N and Ntoumi F (2005). In vivo chloroquine resistance and prevalence of the pfcrt codon 76 mutation in Plasmodium falciparum isolates from the Republic of Congo. Acta Tropica, 95:219-225.
Ndounga M, Tahar R, Bosko LK, Casimiro PN, Malonga DA and Ntoumi F (2005). Therapeutic efficacy of sulfadoxinepyrimethamine and the prevalence of molecular markers of resistance in under 5 year olds in Brazzaville, Congo. Tropical Medicine and International Health, 12:1164-1171
Olawande O (2017). Eleven years after years after ban, Chloroquinen still used for malaria treatment in Nigeria. Premium Times. R e t r i e v e d f r o m https://www.premiumtimesng.com/health/h ealth- features/227663-investigation-elevenyears-ban-chloroquine-still-used-malariatreatment -nigeria.html.
Sidhu AB, Verdier-Pinard D, and Fidock DA (2002). Chloroquine resistance in Plasmodium falciparum malaria parasites conferred by Pfcrt mutations. Science, 298: 210-213.
Lo AC, Faye B, Ba E,Cisse B,Tine R, Abiola A, Ndiaye D, Ndiaye JLA, Ndiaye D, Sokhna C Gomis JF, Dieng Y, Faye O, Ndir O, Miligan P, Cairns M, Hallet R, Sutherland C & Gaye O. (2013). Prevalence of molecular markers of drug resistance in an area of seasonal malaria chemoprevention in children in Senegal. Malaria Journal, 12:137.
FMOH: National Antimalaria Treatment Policy. 2005, Abuja Nigeria: National Malaria and Vector Control Division, Federal Ministry of Health.
Maslachah L, Dachlan YP, Nidom CA and Fitri LE (2017). Experimental models point mutations in Plasmodium falciparum pfatpase6 gene exposed to recurring artemisinin in vitro. Veterinary Medicine International Conference, 422-435.
Jung M, Kim H, Nam KY, and No KT. (2005). Three-dimensional structure of Plasmodium falciparum Ca2+ - ATPase (PfATPase6) and docking of artemisinin derivatives to PfATPase6. Bioorganic and Medical Chemistry Letter, 15: 2994-2997.
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