Molecular Characterization of Radix natalensis (Krauss, 1848) from Gombe and Plateau States, Nigeria

Authors

  • J. Luka Department of Veterinary Parasitology and Entomology, Faculty of Veterinary Medicine, University of Maiduguri, P. M. B 1069, Maiduguri, Nigeria
  • O. J. Ajanusi Department of Veterinary Parasitology and Entomology, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria- Nigeria
  • N. P. Chiezey Animal Reproduction Research Programme, National Animal Production Research Institute (NAPRI), Shika, Zaria-Nigeria
  • J. O. O. Bale Animal Reproduction Research Programme, National Animal Production Research Institute (NAPRI), Shika, Zaria-Nigeria
  • J. T. Tanko Parasitology Division, National Veterinary Research Institute, Vom-Nigeria Corresponding Author’s email: joshuadiriki@yahoo.com

DOI:

https://doi.org/10.4314/njpar.v45i1.5

Keywords:

Nigeria, Molecular, Radix, Characterization

Abstract

Radix (Lymnaea) natalensis (Krauss, 1848) is an essential host in the completion of the life cycle of Fasciola gigantica especially in large areas of Africa, where it is reputed to serve as the main, if not the only intermediate of Fasciola. In this study, 18S rDNA gene was employed for the molecular characterization of morphologically identified Radix natalensis collected from Nigerian Northern cities of Gombe and Jos. The obtained result revealed that the amplification of the 18SrDNA gene at 450bp identified and confirmed the collected species as Radix natalensis. BLASTn search showed that sequences of Nigerian isolates of Radix natalensis had high (99-100%) degree of similarities with each other and with other sequence deposits in the NCBI GenBank. The Nigerian sequences on phylogenetic analysis formed a separate clade distinct from other sequences of Radix natalensis from other parts of Africa and the rest of the world, suggestive of clustering based on geographical location. Thus, the availability and confirmation of Radix (Lymnaea) natalensis from the study locations as evidenced by the results of this study further justifies the assertion of Lymnaea natalensis, being a putative intermediate host of Fasciola spp. in Nigeria. 

Author Biography

J. Luka, Department of Veterinary Parasitology and Entomology, Faculty of Veterinary Medicine, University of Maiduguri, P. M. B 1069, Maiduguri, Nigeria

Department of Veterinary Parasitology and Entomology, Faculty of Veterinary Medicine, Ahmadu Bello University, Zaria- Nigeria

References

Van Damme, D. (2017) Radix natalensis. (2016). The IUCN Red List of Threatened Species 2017: e.T165761A110769214. http://dx.doi.org/10.2305/IUCN.UK.2017-1.RLTS.T165761A110769214.en on 27thJune 2017.

Correa, A. C., Escobar, J. S., Durand, P., Renaud, F., David, P., Jarne, P., Pointier, J-P.and Hurtrez-Boussès, S. (2010). Bridging gaps in the molecular phylogeny of the Lymnaeidae (Gastropoda: Pulmonata), vectors of Fascioliasis. BMC Evolutionary Biology, 10: 381. https://doi.org/10.1186/1471-2148-10-381.

Brown, D. (2005). Freshwater snails of Africa and their medical importance. London: Taylor and Francis.

Falade, M. O. and Otarigho, B. (2015). Shell morphology of three medical important tropical freshwater pulmonate snails from five sites in Southwestern Nigeria. International Journal of Zoological Research, 11: 140 - 150.https://doi.org/10.3923/ijzr.2015.140.150.

Lu, X-T., Gu, Q-Y., Limpanont, Y., Song, L-G., Wu, Z-D., Okanurak, W. and Lv, Z- Y. (2018). Snail-borne parasitic diseases: An update on global epidemiological distribution, transmission interruption and control methods. Infectious Disease of Poverty, 7(1): 28. https://doi.org/10.1186/s40249-018-0414-7.

Haridy, F. M., Morsy, T. A., Gawish, N. I., Antonios, T. N. and Abdel-Gawad, A. G. (2002). The potential reservoir role of donkeys and horses in zoonotic fascioliasis in Gharbia Governorate, Egypt. Journal of Egyptian Society of Parasitology, 32:561- 570.

Mas-Coma, S., Valero, M. A. and Bargues, M. D. (2009). Fasciola, lymnaeids and human fascioliasis, with a global overview on disease transmission, epidemiology, evolutionary genetics, molecular epidemiology and control. Advanced Parasitology, 69:41–146. https://doi.org/10.1016/S0065-308X(09)69002-3

Adedokun, O. A., Ayinmode, A. B. and Fagbemi, B. O. (2008). A comparative study of three methods for detecting Fasciola infections in Nigerian cattle. Veterinarski Arhiv, 78(5):411-416.

Gboeloh, L. B. (2012). Seasonal prevalence of Fasciola gigantica in slaughter cattle in major abattoirs in Port Harcourt. Advances in Agriculture, Sciences and Engineering Research, 2(9): 336-340.

Magaji, A. A., Ibrahim, K., Salihu, M. D., Saulawa, M. A., Mohammed, A. A. and Musawa, A. I. (2014). Prevalence of fascioliasis in cattle slaughtered in Sokoto metropolitan abattoir, Sokoto, Nigeria. Advances in Epidemiology, 2014, 1-5. https://doi.org/10.1155/2014/247258.

Omowaye, S. O., Idachaba, O. S. And Falola, O. O. (2012). The Prevalence of parasitic infections in bile of cattle slaughtered in Jos abattoir, Plateau State, Nigeria. Global Journal of Bioscience and Technology, 1(1):121-123.

Adang, L. K., Kela, S. L. and Kashere, M. B. (2015). Fascioliasis in cattle slaughtered at Gombe abattoir, Nigeria. International Journal of Biological and Chemical Science, (2):783-792.

Howell, A., Mugisha, L., Davies, J., Lacourse, E. J., Claridge, J., Williams, D. J. L., Kelly-Hope, L., Betson, M., Kabatereine, N. B. and Stothard, J. R.

(2012). Bovine fasciolosis at increasing altitudes: Parasitological and malacological sampling on the slopes of Mount `Elgon, Uganda. Parasites and Vectors, 5:196. doi.org/10.1186/1756-3305-5-196.

Malatji, M. P., Lamb, J. M. and Mukaratirwa, S. (2019). Molecular characterization of liver fluke intermediate host lymnaeids (Gastropoda: Pulmonata) snails from selected regions of Okavango Delta of Botswana, KwaZulu-Natal and Mpumalanga provinces of South Africa.

Veterinary Parasitology: Regional Studies and Reports, 17: 100318. doi: 10.1016/j.vprsr.2019.100318.

Carvalho, O. S., Cardoso, P. C. M., Lira, P. M., Rumi, A., Roche, A., Berne, E., Müller, G. and Caldeira, R. L. (2004). The use of the polymerase chain reaction and restriction fragment length polymorphism technique associated with the classical morphology for characterization of

Lymnaea columella, L. viatrix, and L. diaphana (Mollusca: Lymnaeidae). Memorias do Instituto Oswaldo Cruz, 99(5), 503–507. https://doi.org/10.1590/s0074-02762004000500008

Bargues, M. D., Artigas, P., Khoubbane, M., Ortiz, P., Naquira, C. and Mas-coma, S. (2012).Molecular characterization of Galba truncatula, Lymnaea neotropica and L. schirazensis from Cajamarca, Peru and their potential role in transmission of human and animal fascioliasis. Parasites and Vectors, 5:174. https://doi.org/10.1186/1756-3305-5-174

Dung, B.T., Doanh, P. N., The, D.T., Loan, H. T., Losson, B. and Caron, Y. (2013).Morphological and molecular characterization of lymnaeid snails and their potential role in transmission of Fasciola spp. in Vietnam. The Korean Journal of Parasitology, 51(6), 657–662. https://doi.org/10.3347/kjp.2013.51.6.657

Stothard, J. R. , Breâmond, P., Andriamaro, L., Loxton, N. J., Sellin, B., Sellin, E. and Rollinson, D. (2000). Molecular characterization of the freshwater snail Lymnaea natalensis (Gastropoda: Lymnaeidae) on Madagascar with an observation of an unusual polymorphism in

ribosomal small subunit genes. Journal of Zoological Society of London, 252:303-315. https://doi.org/10.1111/j.1469- 7998.2000.tb00625.x

El-Khayat, H. M. M., Mahmoud, K. M. A., Abdel-Hamid, H. and El-Einin, H. M. A. (2015). Application of inter simple sequence repeat (ISSR) rDNA in detecting genetic variation in Lymnaea natalensis snails from certain Egyptian Governorates. African Journal of Biotechnology, 14:1354-1363.https://doi.org/10.5897/AJB2014.14139.

Lawal-Adebowale, O. A. (2012) Dynamics of ruminant livestock management in the context of the Nigerian agricultural system. In Agricultural and Biological Sciences “Livestock production” edited by Khalid Javed. IntechOpen, pp. 61-80. https://doi.org/10.5772/5292.

Sule, S., Abashiya, M. and Iliya, M. (2021). Analysis of water quality of small scale irrigation scheme in Kwadon, Yamaltu- Deba LGA of Gombe State, Nigeria. International Journal of Environmental Quality, 41:33-39. https://doi.org/10.6092/issn.2281- 4485/11166.

Agada, G. A. A., Abdullahi, I. O., Aminu,M., Odugbo, M., Chollom, S. C., Okeke, L. A. and Okwori, A. E. J. (2014). Prevalence and risk factors associated with Salmonella species contamination of commercial poultry farms in Jos, Plateau State, Nigeria. World Journal of Biology and

Biological Sciences, 2:049-061.

Pam, V. A., Dakul, D. A., Karshima, N. S.,Bata, S. I., Ogbu, K. I., Daniel, L. N., Udokaninyene, A. D., Kemza, S. Y., Igeh, C. P. and Hassan, A. A. (2013). Survey of Cryptosporidium species among ruminants in Jos, Plateau State, North-Central Nigeria. Journal of Veterinary Advances, 3(2): 49-54

Sharif, M., Daryani, A. and Karimi, S. A. (2010). A faunistic Survey of cercariae isolated from lymnaeid snails in central areas of Mazandaran, Iran. Pakistan Journal of Biological Science, 13:158-163.

Tamura, K., Stecher, G., Peterson, D., Filipski, A. and Kumar, S. (2013). MEGA 6: Molecular Evolutionary Genetic analysis version 6.0. Molecular Biology and Evolution, 30: 2725–2729. https://doi.org/10.1093/molbev/mst197.

Altschul, S. F., Gish, W., Miller, W., Myers, E. W. and Lipman, D. J. (1990). Basic local alignment search tool. Journal of Molecular Biology, 215: 403 - 410. https://doi.org/10.1016/S0022-2836(05)80360-2.

Opperdoes, F. (2004). Construction of a distance tree using clustering with the unweighted pair group method with arithmetic mean (UPGMA). Online publication. http://www.icp.ucl.ac.be/~opperd/private/upgma.html

Bargues, M. D. and Mas-coma, S. (1997). Phylogenetic analysis of Lymnaeid snails based on 18S rDNA sequences. Molecular Biology and Evolution, 14:569-577. https://doi.org/10.1093/oxfordjournals.mol bev.a025794.

Okpala, H. O., Ajayi, J. A., James-Rugu, N. N. and Emelike, O. F. (2010). Survey of snail intermediate hosts of trematodes in Jos south Local Government area, Plateau State, Nigeria. International Journal of Natural and Applied Sciences, 6(2):151-154.

Danladi, S. I., Istifanus, W. A. and Samaila, A. B. (2019). The freshwater snail fauna of the Dadin-Kowa man-made reservoir, Gombe State. Integrated Journal of Fauna and Biological Studies, 6(5):31- 35.

Published

2024-04-02

How to Cite

Luka, J., Ajanusi, O. J., Chiezey, N. P., Bale, J. O. O., & Tanko, J. T. (2024). Molecular Characterization of Radix natalensis (Krauss, 1848) from Gombe and Plateau States, Nigeria. Nigerian Journal of Parasitology, 45(1), 39–46. https://doi.org/10.4314/njpar.v45i1.5

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