Enhancing Pediculicidal Activity Against <i>Pediculus humanus capitis</i> Using Iron Oxide Nanoparticle-Based Formulations of some Plant Extracts and Acetic Acid Solution

Authors

  • J. M. Zainab Department of Biology, College of Science, University of Misan, Iraq.
  • K. Sahira Ministry of Education, Baghdad Education Directorate.
  • A. K. Al-Abboodi Department of Biology, College of Science, University of Misan, Iraq.
  • H. A. Alsaady Department of Biology, College of Science, University of Misan, Iraq.

DOI:

https://doi.org/10.4314/njpar.v45i2.21

Keywords:

Fe3O4, Pediculus humanus capitis, Azadirachta indica, Nigella sativa, Sesamum indicum, acetic acid, pediculicides, nanoparticlebased formulation

Abstract

Infestations of head lice (Pediculus humanus capitis) continue to be a major public health concern. There have been instances of lice developing resistance to traditional chemical pediculicides. Therefore, there is a critical need to identify new and effective alternatives. The purpose of this study was to use a filter paper contact bioassay to determine the toxicity of three plant extracts (Azadirachta indica, Nigella sativa, and Sesamum indicum) on head lice, both alone and in combination with iron oxide nanoparticles (Fe3O4 Nps), at a concentration of 202mg L-1. The pediculicidal activity of A. indica oil was 88% at 3 μl/cm , whereas that of acetic acid solution (white vinegar) was 81% within 12 h, both without nanoparticles. With the addition of iron oxide nanoparticles, the toxicity was significantly amplified, and within 6-12 hours, A. indica and acetic acid killed all lice. When exposed to Fe3O4 nanoparticles, N. sativa died after 12 h. When exposed to Fe3O4 nanoparticles, S. indicum exhibited moderate toxicity (35% mortality) compared with its harmless effects. Within 12 h, A. indica eradicated all lice at a concentration of 6 μl/cm2, whereas an acetic acid solution resulted in 82% mortality. A. indica mixed with Fe3O4 nanoparticles, caused complete death within 1h and with acetic acid within 6h. N. sativa exhibited a death rate of 72% when exposed to Fe3O4 nanoparticles at 12 h. These results show that formulations based on iron oxide nanoparticles, combined with A. indica and acetic acid, have great promise as pediculicidal agents to fight head lice infestations. 

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References

Lymbery, A. J., & Smit, N. J. (2023). Conservation of parasites: a primer. International Journal for Parasitology: Parasites and Wildlife. DOI: 10.1016/j.ijppaw.2023.07.001.

Jamil, M., Idrees, A., Qadir, Z. A., Elahi, M. E., Imran, F., Qasim, M., & Sadia, B. I. (2022). Medical and Veterinary Ectoparasites' Importance: An Insight on Alternative Control. Pakistan Journal of Medical and Health Sciences, 16(01), 667. DOI: 10.53350/pjmhs22161667.

Mehlhorn, H. (2011). Head lice and their control: A never-ending story. In Handbook of Hair in Health and Disease (pp. 353-385). WageningenAcademic. DOI: 10.3920/978-90-8686-728-8_18.

Trüeb, R. M., Gavazzoni Dias, M. F. R., & Dutra Rezende, H. (2023). Parasitic Diseases and Infestations of the Hair and Scalp. In Hair in Infectious Disease: Recognition, Treatment, and Prevention (pp. 261-290). Cham: Springer International Publishing. DOI: 10.1007/978-3-031-30754-6_8.

Feldmeier, H. (2023). Head lice as vectors of pathogenic microorganisms. Tropical Medicine and Health, 51(1), 53. DOI: 10.1186/s41182-023-00545-5.

Lamassiaude, N., Toubate, B., Neveu, C., Charnet, P., Dupuy, C., Debierre-Grockiego, F., & Charvet, C. L. (2021). The molecular targets of ivermectin and lotilaner in the human louse Pediculus humanus humanus: New prospects for the treatment of pediculosis. PLoS Pathogens, 17(2), e1008863. DOI: 10.1371/journal.ppat.1008863.

Fox, K., Larkin, K., & Sanchez, A. (2020). Global trends in genetic markers of Pediculus humanus capitis resistance mechanisms. Current Tropical Medicine Reports, 7, 65-73. DOI: 10.1007/s40475-020-00204-3.

Abbasi, E., Daliri, S., Yazdani, Z., Mohseni, S., Mohammadyan, G., Hosseini, S. N. S., & Haghighi, R. N. (2023). Evaluation of resistance of human head lice to pyrethroid insecticides: a meta-analysis study. Heliyon, 9(6). DOI: 10.1016/j.heliyon.2023.e17219.

Choi, J. Y., Boo, M. Y., & Boo, Y. C. (2024). Can Plant Extracts Help Prevent Hair Loss or Promote Hair Growth? A Review Comparing Their Therapeutic Efficacies, Phytochemical Components, and Modulatory Targets. Molecules, 29(10), 2288. DOI: 10.3390/molecules29102288.

Lawi, Z. K. K., Merza, F. A., Banoon, S. R., Jabber Al-Saady, M. A. A., & Al-Abboodi, A. (2021). Mechanisms of antioxidant actions and their role in many human diseases: A review. Journal of Chemical Health Risks, 11. DOI: 10.22034/jchr.2021.683158.

Inglis, K. (2012).Ayurveda:Asian Secrets of Wellness, Beauty and Balance. Tuttle Publishing.

Ascher, K. S. (1993). Nonconventional insecticidal effects of pesticides available from the neem tree, Azadirachta indica. Archives of insect Biochemistry and Physiology, 22(3-4), 433-449. DOI: 10.1002/arch.940220311.

Jones, R., & Crow, J. (2012). The Little Book of Nits.A&C Black.

Vahitha, V., Lali, G., Prasad, S., Karuppiah, P., Karunakaran, G., &AlSalhi, M. S. (2024). Unveiling the therapeutic potential of thymol from Nigella sativa L. seed: selective anticancer action against human breast cancer cells (MCF-7) through down-regulation of Cyclin D1 and proliferative cell nuclear antigen (PCNA) expressions. Molecular Biology Reports, 51(1), 61. DOI: 10.1007/s11033-023-09032-w.

Ahmed, S. A., A. Nagadi, S., Abo-Elyousr, K. A., & El-Fawy, M. M. (2024). Mitigating helminthosporium leaf spot disease in sesame: evaluating the efficacy of castor essential oil and sodium bicarbonate on disease management and crop yield enhancement. Journal of Plant Pathology, 1-12. DOI: 10.1007/s42161-024- 01612-4.

Soonwera, M. (2014). Efficacy of herbal shampoo based on native plant against head lice (Pediculus humanus capitis De Geer, Pediculidae: Phthiraptera) in vitro and in vivo in Thailand. Parasitology Research, 113(9), 3241-3250. DOI: 10.1007/s00436-014-3986-6.

Al-Abboodi, A., Alsaady, H. A. M., Banoon, S. R., & Al-Saady, M. (2021). Conjugation strategies on functionalized iron oxide nanoparticles as a malaria vaccine delivery system. Revista Bionatura, 6(3), 2009- 2015. DOI: 10.21931/RB/2021.06.03.20.

Al-Abboodi, A., Albukhaty, S., Sulaiman, G. M., Al-Saady, M. A., Jabir, M. S., & Abomughaid, M. M. (2024). Protein-conjugated superparamagnetic iron oxide nanoparticles for efficient vaccine delivery systems. Plasmonics, 19(1), 379-388. DOI: 10.1007/s11468-023-01994-8.

Ali, Z. H.,Al-Saady, M.A.A. J.,Aldujaili, N. H., Rabeea Banoon, S., &Abboodi,A. (2022). Evaluation of the Antibacterial Inhibitory Activity of Chitosan Nanoparticles Biosynthesized by Streptococcus thermophilus. Journal of Nanostructures, 12(3), 675-685. DOI: 10.22052/JNS.2022.03.020.

Alsaady, H. A. M., Al-Abboodi, A., Awdaa, A., & Rshak, M. (2020). The Infestation Study of Oestrus ovis L. 1761 in Sheep of Al-Amara Region, Maysan Province, South of Iraq. Indian Journal of Forensic Medicine & Toxicology, 14(2), 1180-1187. DOI: 10.37506/ijfmt.v14i2.3066.

Alsaady, H. A. M., Aswan, A. A., & Abbood, E. S. (2021). Sero-epidemiology of Toxoplasma gondii among men and pregnant women in Maysan Province, south of Iraq. Iranian Journal of Ichthyology, 8, 27-37. DOI: 10.24017/science.2020.ICHMS2020.8.

Penkar, G. M., Salkar, M. R., Chavan, P. S., Ambade, M. S., Parab, S. A., Padte, T. S., & Jagtap, V. A. (2023). An overview on Indian herbs in hair care therapy. Research Journal of Pharmacognosy and Phytochemistry, 15(2), 161-166. DOI: 10.52711/0975-4385.2023.00025.

Nunez, C., Bamert, R. S., Lambert, K., & Short, F. L. (2024). Cleaning Up Our Disinfectants: Usage of antimicrobial biocides in direct-to-consumer products in Australia. Access Microbiology, 6(2), 000714-v3. DOI: 10.1099/acmi.0.000714.v3.

Ganchev, D. (2023).Antifungal effect of several organic acids towards conidiospores of monilia fructigena in Zinab et al: Enhancing Pediculicidal Activity against Pediculus humanus capitis 468 the in vitro conditions. Knowledge-International Journal, 59(3), 213-217. DOI: 10.21608/jacb.2012.55011.

Malik,A. Q., Mir, T. U. G., Kumar, D., Mir, I.A., Rashid,A.,Ayoub, M., & Shukla, S. (2023).Areview on the green synthesis of nanoparticles, their biological applications, and photocatalytic efficiency against environmental toxins. Environmental Science and Pollution Research, 30(27), 69796-69823. DOI: 10.1007/s11356-023-27437-9.

Radwan, I. T., Eltaly, R. I., Baz, M. M., Yousif, M., Selim, A., Taie, H. A., & Khater, H. F. (2023). Novel acaricidal and growth-regulating activity of Aloe vera and Rheum rhabarbarum extracts and their oil/water nanoemulsions against the camel tick, Hyalomma dromedarii. Scientific Reports, 13(1), 16802. DOI: 10.1038/s41598-023-43776-6.

Radwan, I. T., Khater, H. F., Mohammed, S. H., Khalil,A., Farghali, M.A., Mahmoud, M. G., & Baz, M.. (2024). Synthesis of eco-friendly layered double hydroxide and nanoemulsion for jasmine and peppermint oils and their larvicidal activities against Culex pipiens Linnaeus. Scientific Reports, 14(1), 6884. DOI: 10.1038/s41598-024-56802-y.

Manna, S., Roy, S., Dolai,A., Ravula,A. R., Perumal, V., & Das,A. (2023). Current and future prospects of “all-organic” nanoinsecticides for agricultural insect pest management. Frontiers in Nanotechnology, 4, 1082128. DOI: 10.3389/fnano.2022.1082128.

Gatti, A. M., & Montanari, S. (2015). Case studies in nanotoxicology and particle toxicology. Academic Press. DOI: 10.1016/C2013-0-18692-8.

Chaudhary, P., Sharma, R., Rawat, S., & Janmeda, P. (2023). Antipyretic medicinal plants, phytocompounds, and green nanoparticles: an updated review. Current Pharmaceutical Biotechnology, 24(1), 23-49. DOI: 10.2174/1389201023666220330005020.

Onen, H., Luzala, M. M., Kigozi, S., Sikumbili, R. M., Muanga, C. J. K., Zola, E. N., & Memvanga, P. B.(2023). Mosquito-borne diseases and their control strategies: an overview focused on green synthesized plantbased metallic nanoparticles. Insects, 14(3), 221. DOI: 10.3390/insects14030221.

Moraes-de-Souza, I., de Moraes, B. P., Silva,A. R., Ferrarini, S. R., & Gonçalves-de-Albuquerque, C. F. (2024). Tiny Green Army: Fighting Malaria with Plants and Nanotechnology. Pharmaceutics, 16(6), 699. DOI: 10.3390/pharmaceutics16060699.

Published

2024-09-23

How to Cite

Zainab, J. M., Sahira, K., Al-Abboodi, A. K., & Alsaady, H. A. (2024). Enhancing Pediculicidal Activity Against <i>Pediculus humanus capitis</i> Using Iron Oxide Nanoparticle-Based Formulations of some Plant Extracts and Acetic Acid Solution. Nigerian Journal of Parasitology, 45(2), 460–469. https://doi.org/10.4314/njpar.v45i2.21

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