Serological and molecular survey of Trypanosoma evansi in Camels (Camelus dromedarius) in Maiduguri, North-east, Nigeria
https://dx.doi.org/10.4314/njpar.v42i1.8
Keywords:
Serology, PCR, Dromedary camel, T.evansi, MaiduguriAbstract
To date, camels still remain an important work animal as well as source of protein to humans in the Sudan and Sahel regions of Nigeria. Therefore, a cross-sectional study was conducted on 150 camels slaughtered in Maiduguri central abattoir to determine the prevalence of Trypanosoma evansi using Card Agglutination Test (CATT) and Polymerase Chain Reaction (PCR) techniques. Overall, 30 (20%) of the camels tested were seropositive while PCR targeting the 227 base pair of the Variable Surface Glycoprotein (VSG) gene of T. evansi detected the DNA of the parasite in 9 out of the 30seropositive camels. Higher infection was found among adult compared to the young camels using the two diagnostic techniques; 24.1% vs 19.0% and 10.3% vs 4.6%, for CATT and PCR techniques, respectively. However, the differences being not statistically significant (P > 0.05) for the two methods of diagnosis. Furthermore, significantly (P < 0.05)higher prevalence of infection was recorded among male compared to female camels using the serological method of diagnosis, while (P > 0.05) using the molecular method; 27.5% vs 13.6% for CATT and 10.1% vs 2.5% for PCR. Camels with PCV =24 %( mean: 19.8923 ± 4.0931) recorded significantly (P < 0.05) higher prevalence of 23.1% than those with PCV = 25% (mean 31.7294 ± 5.50584), where the prevalence was 17.6%.The results of this study showed that camel trypanosomosis is endemic in the study area. Further studies to elucidate the epidemiology and socioeconomic impact of this disease in the northeast region of Nigeria are desirable.
References
Faye, B. (2016). The camel new challenges for a sustainable development. Tropical Animal Health and Production, 48(4),689- 692.
Anas, S. Q.,Muzaffar, G.,Farrah, D. and Qamar-un, N. (2018). Prevalence of haemoparasite of camels (Camelus Dromedaries) in Thal Desert Pakistan in winter. EC. Veterinary Science, 3(1),246- 249.
Jilo, K. (2017). “Camel Trypanosomiasis: A review on past and recent research in Africa and Middle east”. AmericanEurasian Journal of scientific Research, 12(1), 13-20.
Zelalem, A., Akil, U. and Zelalem, A. (2015). Review on Camel Trypanosomosis: Its Epidemiology and Economic Importance. Acta Parasitologica Globalis, 6(2), 117-128.
Luckins, A.G. (1998). Epidemiology of surra: Unanswered questions. Journal of Protozoology Research. 8:106-19
World Organization for Animal Health (OIE) (2012). Trypanosoma evansi Infection (Surra). Version adopted by world assembly of delegates of the OIE in May 2012.
Buscher, P., Gonzatti, M.I., Hebert, L., Inoue, N., Pascucci, I., Schnaufer, A., Suganuma, K., Touratier, L. and Reet, N.( 2019).Equine Trypanosomosis: Enigmas and diagnostic challenges. Parasites and Vectors, 12(234)
Desquesnes, M., Biteau-Coroller, F., Bouyer, J., Dia, M.L., and Foil, L.(2009). Development of a Mathematical Model for Mechanical Transmission of Trypanosomes and Other Pathogens of Cattle Transmitted by Tabanids. International Journal for Parasitology. 39:333�346.
Diall, O., Bajyana, S.E., Magnus, E., Kauyate, B.,Diallo, B.,Van, M.,Venne, N.,and Hamers, R.( 1994).Evaluation of a direct serologic card agglutination test for the diagnosis of camel Trypanosomiasis caused by Trypanosoma evansi. Revue Scientifique et Technique, 13(3): 793-800
Holland, W.G., Thanh, N.G., Do, T.T., and Sangmaneedet, J. (2005).Evaluation of diagnostic tests for Trypanosoma evansi in experimentally infected pigs and subsequent use in field surveys in North Vietnam and Thailand. Tropical Animal Health and Production, 37(6), 457-467
Reid, S.A., and Cope man, D.B.( 2003). The development and validation of an-antibodyELISA to detect Trypanosoma evansi infection in cattle in Australia and Papua New Guinea. Preventive Veterinary Medicine, 61(3), 195-208
Verloo, D., Holland, W., Myc, L.N., Thanh, N.G., Tamc, P.T., Goddeeris, B., Vercruysse, J., and Bü scher, P. (2000). Comparison of Serological Tests for Trypanosoma evansi Natural Infections in Water Buffaloes from North Vietnam. Veterinary Parasitology, 92, 87-96.
Clausen, P.H., Wiermann,A.,Patzelt, R., Kakaire, D.,Poetzsch, C.,and Peregrine, A.( 1998). Use of a PCR assay for the specific and sensitive detection of Trypanosoma spp. In naturally infected dairy cattle in periurban Kampala, Uganda. Ann. N. Y. Academic Science. 849:21-23
Sengupta, P. P., Balumahendiran, M., Suryanarayana, V. V. S., Raghavendra, A. G., Shome, B. R.,Ganjendragad, M. R. and Prabhudas, K. (2010). PCR-Based Diagnosis of Surra-Targeting VSG Gene:Experimental Studies in Small Laboratory Rodents and Buffalo. Veterinary Parasitology, 171, 22-31.
Musa, A.W and Pindar, T.Y. (2005). Geographical history of Borno State, by the Borno state ministry for local government and chieftaincy affairs. ALGON Diary.
Bello, A.,Sonfada M. L.,Umar, A. A., Umaru, M. A., Shehu, S. A., Hena, S. A., Onu, J.E. and Fatima, O.O. (2013). Age estimation of camel in
Nigeria using rostral dentition. Science Journal of Animal Science, 2(1), 9- 14.
Thrusfield, M. V. (1995). Veterinary Epidemiology, (2nded). Blackwell publishing. Oxford Greet Britain
Woo, P.T.K.,(1970). The haematocrit centrifuge technique for diagnosis of African Trypanosomiasis. ActaTropica 27, 384-386
Wuyts, N., Chodesajjawatee, N. and Panyim, S. ( 1994). A simplified and highly sensitive detection of Trypanosoma evansi by DNA amplification. Southeast Asian Journal of Tropical Medicine Public Health, 25, 266- 271.
Birhanu, H., Fikru,R., Said, M., Kidane, W., Gebrehiwot, T., Hagos, A., Alemu, T., Dawit, T.,Berkvens, D.,Goddeeris, M.B.,and Buscher, P.(2015). Epidemiology of Trypanosoma evansi and Trypanosoma vivax in domestic animals from selected districts of Tigray and Afar regions, Northern Ethiopi a. Parasites & Vectors.8:212
Aregawi, W.G., Kass, S.T., Tarekgn, K.D., Birhanu, W.T., Haile, S.T. and Kiflewahid, F.W. (2015). Parasitological and Serological study of camel Trypanosomosis (Surra) and associated risk factors in Gabi Rasu Zone, Afar, Ethiopia, Journal of Veterinary Medicine and Animal Health. 7(6): 234-240
Mohamoud, A.H.I. (2017) Sero-Prevalence Study of Camel Trypanosomiasis in Selected Villages of Galkayo, Somalia. Open Journal of Veterinary Medicine, 7, 31- 37.
Tehseen, S.,Johan, N.,Qamar, M. F., Desquesnes, M., Shahzad, M. I., Deborggroev, S.,and Buscher, P. (2015). Parasitological, Serological and Molecular survey of Trypanosoma evansi infection in dromedary camels from Cholistan Desert, Pakistan. Parasites& Vectors, 8, 1-1
Barry, J. D., Marcello, L., Morrison, L. J., Read, A. F.,Lythgoe, K.,Jones, N., Carrington, M.,Blandin, G.,Bohme, U., Caler, E., Hertz-Fowler, C., Renauld, H., ElSayed, N. and Berriman, M.( 2005) What the genome sequence is revealing about trypanosome antigenic variation. Biochemical. Society. Transactions.33: 986-989
Pays, E.Vanhamme, L. and Pe´ Rez-Morga, D . (2004) Antigenic variation in Trypanosoma brucei: Facts, challenges and mysteries. Curr. Op. Microbiol. 7, 369–374.
Atarhouch, T., Rami, M., Bendahman, M.N. and Dakkak, N.A.(2003). Camel Trypanosomosis in Morocco. Results of a first epidemiological survey, Veterinary Parasitology, 111: 277-286.
Pathak, K.M.L. and Khanna, N.D. (1995). Trypanosomosis in Camel (Camelus dromedarius) with Particular Reference to Indian Subcontinent: A Review. International Journal of Animal Sciences, 10, 157-162.
Bala, E.A., Abakar, D.A., Mohammed, S.M. and Abbas, A.M. (2018). Prevalence of Trypanosoma evansi in camels in four states of great Butana, Sudan. International Journal of Zoology Studies. 3(3): 33-37.
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