The Effect of <i>Trichomonas vaginalis</i> and some Urogenital Bacteria on Male Fertility in Maysan Province, Iraq
DOI:
https://doi.org/10.4314/njpar.v45i2.10Keywords:
Trichomonas vaginalis, bacteria, semen, urine, males, Maysan IraqAbstract
Trichomonas vaginalis and associated bacteria are important urogenital pathogens. This study aimed to investigate the effect of T. vaginalis and its associated bacteria on semen and sperm parameters related to male fertility. Urine and semen samples were collected from 97 males, whose ages between 18-50 years. Semen and sperm parameters, such as semen volume, sperm count, sperm
concentration, sperm motility, leucocytes/pus cells, and erythrocytes, were evaluated according to the WHO instructions. Conventional- PCR and real-time quantitative PCR were used to detect T. vaginalis infection. Amie's-transport, blood-agar, MacConkey-agar, Chocolate-agar, Thayer-Martin-agar, and Mannitol-salt-agar were used to culture bacteria in urine, and the Vitek2 system
was used to differentiate bacteria. Samples that showed positive microscopic examination for bacteria but were not culturable were diagnosed using PCR. T.vaginalis and bacterial infection rates were 31.8% and 17.5%, respectively. T. vaginalis, bacteria, and both together caused a decrease in some qualitative and quantitative parameters of semen and sperm such as a decline in sperm total count by 46.5, 63.46, and 65.67% respectively, sperm concentration by 6.27, 15.37, and 44.27% respectively, sperm motility by 67.63, 66.18 and 66.75% respectively, semen volume by 42.2, 36.4 and 63.86% respectively. They caused increases of 100.92, 16.89, and 84.56%, respectively, and sperm death by 36.58, 80.0%, 93%, and 60.77%, respectively. In addition, they caused an increase in WBCs/pus cells
and RBCs in the semen and urine. Four bacteria species, Pantoea spp., Sphingomonas paucimobilis, Staphylococcus aureus, Methylobacterium spp. were detected in urine, and 10 species, Enterobacter aerogenes, Serratia marcescens, Pseudomonas putida, Sphingomonas paucimobilis, Aeromonas sobria, Burkholderia cepacia, Staphylococcus aureus, Aerococcus viridans, Pseudomonas androgens and Staphylococcus epidermidis were detected in semen. In addition, PCR identified three unculturable species: one in urine (Holomonas spp.), and two in semen (Lactobacillus jensenii and Holomonas spp.), S. aureus had the highest frequency in urine and semen of (32%)
References
Guiton, R., & Drevet, J. R. (2023). Viruses, bacteria and parasites: infection of the male genital tract and fertility. Basic and clinical andrology, 33(1): 19. https://doi.org/10.1186/s12610-023-00193 Jabr et al: Effect of Trichomonas vaginalis and some Urogenital Bacteria 346
Rowley, J., Vander Hoorn, S., Korenromp, E., Low, N., Unemo, M., Abu-Raddad, L. J., Chico, R. M., Smolak, A., Newman, L., Gottlieb, S., Thwin, S. S., Broutet, N. & Taylor, M. M. (2019). Chlamydia, gonorrhoea, trichomoniasis and syphilis: global prevalence and incidence estimates, 2016. Bulletin of the World Health Organization, 97(8): 548-562.
Kim, S. J., Paik, D. J., Lee, J.S., Lee, H.S ., Seo, J. T., Jeong, M. S., Lee, J. H., Park, D. W., Han, S., Lee, Y. K., Lee, K. H., Lee, I. H., So, K. A., Kim, S. H. & Kim, T. J. (2017). Effects of infections with five sexually transmitted pathogens on sperm quality. Clinical and Experimental Reproductive Medicine, 44(4): 207-213.
Zorman, J. V., Matičič, M., Jeverica, S. & Smrkolj, T. (2015). Diagnosis and treatment of bacterial prostatitis. Acta Dermatovenerologica Alpina Pannonica et Adriatica, 24(2): 25-29. https://doi:10.15570/actaapa.2015.8
Silea, C., Holban, A. M., & Chifiriuc, M. C. (2018). Role of genital tract infections and inflammatory processes in male infertility. Reviews in Biological and Biomedical Sciences, 1(1) 34-42. https://doi.org/10.31178/rbbs.2018.1.1.5
WHO, World Health Organization. (2012). Global incidence and prevalence of selected curable sexually transmitted infections: 2008, Reproductive Health Matters, 20(40): 207-209, https://doi.org/10.1016/S0968- 8080(12)40660-7
Newman, L., Rowley, J., Vander Hoorn, S., Wijesooriya, N. S., Unemo, M., Low, N., Stevens, G., Gottlieb, S., Kiarie, J. & Temmerman, M. (2015). Global estimates of the prevalence and incidence of four curable sexually transmitted infections in 2012 based on systematic review and global reporting. PloS One, 10(12): e0143304.
Al-Majidii, N. K. S. & Alsaady, H. A. M. (2020). The Prevalence of Trichomonas vaginalis Parasite Among Women in Some Regions of Maysan province, Periódico Tchê Química, 17 (n°36): 784-797.
Parker, N., Schneegurt, M., Tu A-H. T., Frosted, B. M., & Lister, P. (2019). Allied Health Microbiology. Open Education , Oregon State University, Openstax, 1st Ed. 554 pp.
Mielczarek, E., & Blaszkowska, J. (2016). Trichomonas vaginalis: pathogenicity and potential role in human reproductive failure. Infection, 44: 447-458. https://doi:10.1007/s15010-015-0860-0
Zhang, Z., Li, Y., Lu, H., Li, D., Zhang, R., Xie, X., Guo, L., Hao, L., Tian, X., Yang, Z., Wang, S., Mei, X. (2022). A systematic review of the correlation between Trichomonas vaginalis infection and infertility. Acta Tropica, 236: 106693. https://doi.org/10.1016/j.actatropica.2022.106693
Zhang, Z., Li, F., Deng, Y., Li, Y., Sheng, W., Tian, X., Yang, Z., Wang, S., Guo, L., Hao, L. & Mei, X. (2023). Trichomonas vaginalis excretory secretory proteins reduce semen quality and male fertility, Acta Tropica 238: 106794. https://doi.org/10.1016/j.actatropica.2022.106794
Upcroft, P. & Upcroft, J. A. (2001). Drug targets and mechanisms of resistance in the anaerobic protozoa. Clinical Microbiology Reviews, 14(1): 150-164.
Kreisel, K. M., Spicknall, I. H., Gargano, J. W., Lewis, F. M., Lewis, R. M., Markowitz, L. E., Roberts, H., Johnson, A. S., Song, R.,S., Cyr, S. B., Weston, E. J., Torrone, E. A. & Weinstock, H. S. (2021). Sexually transmitted infections among US women and men: prevalence and incidence estimates, 2018. Sexually Transmitted Diseases, 48(4): 208-214.
Khalaf, A. K., Al-Asadi, S. A., Al-Yaaqub, A. J. & Al-Mayah, S. H. (2010). Use PCR technique to detect Trichomonas vaginalis among men in Basrah province. Thi-Qar Medical Journal, 4(2): 29-36.
Al-Kafagy, S. M. & Al-Hadraawy, M. K. (2014). Molecular and immunological study for detection of IL- 6 in men infected with Trichomonas vaginalis parasite in Al-Najaf province,; Iraq. Al-Qadisiyah Medical Journal, 10(18): 144-148.
Al-Quraishi, M. A. (2014). Epidemiology study of Trichomonas vaginalis in Babylon province and the efficiency of Mentha spicata leafs extracts in vivo. Journal of Natural Sciences Research, 4(2): 72-82.
Tsevat, D. G., Wiesenfeld, H. C., Parks, C. & Peipert, J. F. (2017). Sexually transmitted diseases and infertility. American Journal of Obstetrics and Gynecology, 216(1): 1-9.
Eini, F., Kutenaei, M. A., Zareei, F., Dastjerdi, Z. S., Shirzeyli, M. H. & Salehi, E. (2021). Effect of bacterial infection on sperm quality and DNA fragmentation in subfertile men with Leukocytospermia. BMC Molecular and Cell Biology, 22(1): 42. https://doi.org/10.1186/s12860-021-00380-8
Dessì, D., Margarita, V., Cocco, A. R., Marongiu, A., Fiori, P. L. & Rappelli, P. (2019). Trichomonas vaginalis and Mycoplasma hominis: new tales of two old friends. Parasitology, 146(9): 1150-1155.
Barrientos-Durán, A., de Salazar, A., Alvarez-Estévez, M., Fuentes-López, A., Espadafor, B. & Garcia, F. (2020). Detection of sexually transmitted disease-causing pathogens from direct clinical specimens with the multiplex PCR-based STD Direct Flow Chip Kit. European Journal of Clinical Microbiology and Infectious Diseases: Official Publication of the European Society of Clinical Microbiology, 39(2): 235-241.
Caliendo,A. M., Jordan, J.A., Green,A., Ingersoll, J., Diclemente, R. J. & Wingood, G. M. (2005). Realtime PCR improves detection of Trichomonas vaginalis infection compared with culture using self-collected vaginal swabs. Infectious Diseases in Obstetrics and Gynecology, 13(3): 145-150.
Choe, H. S., Lee, D. S., Lee, S. J., Hong, S. H., Park, D. C., Lee, M. K., Kim, T. H. & Cho, Y. H. (2013). Performance of Anyplex™ II multiplex real-time PCR for the diagnosis of seven sexually transmitted infections: comparison with currently available methods. International Journal of Infectious Diseases, 17(12): e1134-e1140.
WHO (2021). Laboratory manual for the examination and processing of human semen, sixth edition. Geneva: World Health Organization;. Licence: CC BY-NC-SA3.0 IGO.
dos Santos, O., de Vargas Rigo, G., Frasson, A.P., Macedo, A.J., & Tasca, T. (2015). Optimal reference genes for gene expression normalization in Trichomonas vaginalis. PLoS One, 10(9): e0138331.
Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). Molecular cloning: a laboratory manual (2nd Ed.), New York, United States: Cold Spring Harbor Laboratory Press.
Srinivasan, R., Karaoz, U., Volegova, M., MacKichan, J., Kato-Maeda, M., Miller, S., Nadarajan, R., Brodie, E. L. & Lynch, S. V. (2015). Use of 16SrRNA gene for identification of a broad range of clinically relevant bacterial pathogens. PloS One, 10(2): e0117617.
Waters, D. L. & Shapter, F. M. (2014). The polymerase chain reaction (PCR): general methods. Cereal Genomics: Methods and Protocols, NewYork, United States: Springer, pp. 65-75.
Ijeoma,A. O.,Amara, V. N. & Emmanuel, I. O. (2018) Prevalence of Trichomoniasis amongAdults in OruEast L.G.A, Imo State, Nigeria. Archives of Clinical Microbiology, 9(2): 79. https://doi.org/10.4172/1989- 8436.100079
Al-Khafaji, S. H., & Ali, Z. M. (2021). Study of Bacteriospermia Isolated from Male Infertility in Al-Najaf City, Iraq. Scientific Journal of Medical Research, 5(19): 85-90.
Shafir, S. C., Sorvillo, F. J., Smith, L. (2009). Current issues and considerations regarding trichomoniasis and human immunodeficiency virus inAfrican–Americans. Clinical Microbiology Reviews, 3(22):37–45.
Roh J., Lim Y. S., Seo M. Y., Choi Y., & Ryu J. S. (2015). The secretory products of Trichomonas vaginalis decrease fertilizing capacity of mice sperm in vitro. Asian Journal of Andrology, 17(2): 319–323. https://doi.org/10.4103/1008-682X.145070
Margarita, V. (2014). Several aspects of pathogenesis of Trichomonas vaginalis. PhD thesis, University of Sassari, Italy.
Rendon-Maldonado, J. G. Espinosa-Cantellano M, González-Robles A, & Martínez-Palomo A. (1998) Trichomonas vaginalis: in vitro phagocytosis of lactobacilli, vaginal epithelial cells, leukocytes, and erythrocytes. Experimental Parasitology, 89(2): 241-250. https://doi.org/10.1006/expr.1998.4297
Benchimol, M. (2004). Trichomonads under Microscopy. Microscopy Microanalysis, 10(5): 528-50. https://doi.org/10.1017/S1431927604040905
Benchimol, M., de Andrade Rosa, I., da Silva Fontes, R., Burta Dias, A. (2008) Trichomonas adhere and phagocytose sperm cells: adhesion seems to be a prominent stage during interaction. Parasitology Research, 102: 597-604.
Gomez, C., Esther Ramirez, M., Calixto-Galvez, M., Medel, O. & Rodríguez, M.A. (2010). Regulation of gene expression in protozoan parasites. Journal of Biomedicine and Biotechnology, 2010. https://doi.org/10.1155/2010/726045.
Lacroix, J. M., Jarvi, K., Batra, S. D., Heritz, D. M., & Mittelman, M.W. (1996). PCR-based technique for the detection of bacteria in semen and urine. Journal of Microbiological Methods, 26(1-2), 61-71.
Ahmadi M. H., Mirsalehian A, Bahador A. G. (2016). Prevalence of Urogenital Mycoplasmas in Iran and Their Effects on Fertility Potential: A Systematic Review and Meta-Analysis. Iran Journal of Public Health 45(4):409-22.
Zuber A., Peric A., Pluchino N., Baud D. & Stojanov M. (2023). Human Male Genital Tract Microbiota. International Journal of Molecular Sciences, 24(8), 6939. https://doi.org/10.3390/ijms24086939
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