Evaluation of Hormones and other Biochemical Parameters among Chronic Liver Disease Patients Infected with <i>Toxoplasma gondii </i>
DOI:
https://doi.org/10.4314/njpar.v45i1.12Keywords:
Toxoplasma gondii, TSH, TGF, Prolactin, IL-13, Cortisol, Chronic liver diseaseAbstract
This study was conducted in Baghdad, Iraq from December 2021 to May 2022. The goal was to determine the effect of
Toxoplasma gondii on liver function by examining the relationship between Toxoplasma infection and hormones. One hundred and twenty male patients with Chronic liver disease (CLD) (age:14-75 years) and 120 control males (age: 24-70 years) participated in this study. Serum samples were taken from all individuals and were then analysed for anti-Toxoplasma antibodies. Hormonal tests were conducted for all participants which included (Cortisol, testosterone, prolactin, insulin, and thyroid-stimulating hormone TSH). Biochemical tests included (Prothrombin time PT, international normalized ratio INR and albumin); liver enzymes evaluated were (aspartate aminotransferase AST, alanine aminotransferase ALT, alkaline phosphatase ALP and gamma-glutamyl transferase GGT) and interleukins (Interleukin 13 IL-13 and transforming growth factor TGF). According to the anti-Toxoplasma antibodies results, 32 (26% of the control group) of the participants tested positive for anti-Toxoplasma antibodies, compared to 60 (50%) of the CLD patients. Four sub-groups were formed in response to prior results: Control-Toxoplasma positive, control-Toxoplasma negative, CLD-Toxoplasma positive and CLDToxoplasma negative. The seropositive status of T. gondii did not affect the following hormones: cortisol, testosterone, insulin and TSH while it was related significantly to prolactin. The results of liver enzymes showed that T. gondii positive status was significantly related to all enzymes among CLD patients except the GGT enzyme T. gondii positive status was not correlated with the other biochemical (PT, INR, and albumin) and immunological parameters (IL-13 and TGF).
References
Jwad, B. A., Al-Haboobi, Z. A., and AlKhafaji, N. M. (2020). Biochemical and hormonal study in women infected with Toxoplasma gondii. Eurasian Journal of Biosciences, 14(1), 515 - 519. https://www.proquest.com/openview/919ba4165c89162de4b12ffc7fe879fe/1.pdf?pqorigsite=gscholar&cbl=2042720
Galvan-Ramirez, M. D. L. L., GutiérrezMaldonado, A. F., Verduzco-Grijalva, F., and Jiménez, J. M. D. (2014). The role of hormones on Toxoplasma gondii infection: a systematic review. Frontiers in Microbiology, 5, 503. https://doi.org/10.3389/fmicb.2014.00503
Shirbazou, S., Abasian, L., and Meymand, F. T. (2011). Effects of Toxoplasma gondii infection on plasma testosterone and cortisol level and stress index on patients referred to Sina Hospital, Tehran. Jundishapur Journal of Microbiology, 4(3), 167 - 173. https://brieflands.com/articles/jjm72449.pdf
Alvarado-Esquivel, C., Torres-Berumen, J. L., Estrada-Martínez, S., Liesenfeld, O., and Mercado-Suarez, M. F. (2011). Toxoplasma gondii infection and liverdisease: a case-control study in a northern Mexican population. Parasites & Vectors, 4(1), 1-7. https://doi.org/10.1186/1756-3305-4-75
Benedetto, N., Folgore, A., RomanoCarratelli, C., and Galdiero, F. (2001). Effects of cytokines and prolactin on the replication of Toxoplasma gondii in murine microglia. European Cytokine Network, 12(2), 348 - 58. http://www.jle.com/medline.md?issn=1148-493&vol=12&iss=2&page=348
Dzitko, K., Gatkowska, J., Płociński, P., Dziadek, B., and Długońska, H. (2010). The effect of prolactin (PRL) on the growth of Toxoplasma gondii tachyzoites in vitro. Parasitology Research, 107(7), 199-204. https://doi.org/10.1007/s00436-010-1849-3.
Anya, M. I., Ozung, P. O., Okah, U., Ayuk, A. A., and Igwe, P. A. (2018). Blood Profile of West African Dwarf (WAD) Goats Fed Cassava Peel Meal Based-Diets Supplemented with African Yambean Concentrate. Canadian Journal ofAgriculture and Crops, 3(2), 55-63. http://dx.doi.org/10.20448/803.3.2.55.63
Salman, Y. J. (2014). Correlation between Toxoplasma gondii and Anti-Mullerian hormone levels in sera of women in Kirkuk City using ELISA method. International Journal of Current Microbiology Applied Sciences, 3(10), 85 - 92. https://www.ijcmas.com/vol-3-10/Yahya%20Jirjees%20Salman.pdf
ay, G., and Bhargav, P. M.(2019). Astudy of hormonal abnormalities in chronic liver disease. Journal of The Association of Physicians of India, 67(4), 47-52.https://pubmed.ncbi.nlm.nih.gov/31311219/
Ghanam, M. E., Shataat, M. A., Mel-S, M., Hassan, A. A., and Younis, A. I. (2001). Evaluation of the role of some parasitic infections as a cause of acute and chronic hepatic diseases. Journal of the Egyptian Society of Parasitology, 31(1), 37-42.https://pubmed.ncbi.nlm.nih.gov/12557927
/
Sarkar, M., VanWagner, L. B., Terry, J. G., Carr, J. J., Rinella, M., Schreiner, P. J., Lewis, C. E., Terrault, N., and Coronary Artery Risk Development in Young Adults (CARDIA) Cohort(2019). Sex Hormone–Binding Globulin Levels in Young Men Are Associated With Nonalcoholic Fatty Liver Disease in Midlife. Official journal of the American College of Gastroenterology, 114(5), 758-7 6 3 .https://doi.org/10.14309%2Fajg.00000000
Mahbodfar, H. R., Yousefi-Razin, E., Saki, J., Rafiei, A., and Khademvatan, S. (2015). Study of latent Toxoplasma gondii role in level of testosterone, DHEA, cortisol and prolactin hormones of young persons. Asian Journal of Epidemiology, 8(3), 64. https://doi.org/10.3923/aje.2015.64.71.
Gluud, C. (1987). Serum testosterone concentrations in men with alcoholic cirrhosis: background for variation. Metabolism, 36(4), 373-378. DOI: 10.1016/0026-0495(87)90210-1.
Van Thiel, D. H., Kumar, S., Gavaler, J. S., and Tarter, R. E. (1990). Effect of liver transplantation on the hypothalamicpituitary-gonadal axis of chronic alcoholic men with advanced liver disease. Alcoholism: Clinical and Experimental Research, 14(1), 478 - 481. https://doi.org/10.1111/j .1530-0277.1990.tb00507.x.
Sinclair, M., Grossmann, M., Hoermann, R., Angus, P. W., and Gow, P. J. (2016). Testosterone therapy increases muscle mass in men with cirrhosis and low testosterone: a randomised controlled trial. Journal of Hepatology, 65(5), 906 - 913. https://doi.org/10.1016/j.jhep.2016.06.007
Schwinge, D., Carambia, A., Quaas, A., Krech, T., Wegscheid, C., Tiegs, G., Prinz, I., Lohse, A. W., Herkel, J., and Schramm, C. (2015). Testosterone suppresses hepatic inflammation by the downregulation of IL17, CXCL-9, and CXCL-10 in a mouse model of experimental acute cholangitis. The Journal of Immunology, 194(6), 2522-2530. DOI: 10.4049/jimmunol.1400076.
Flegr, J., Hrusková, M., Hodný, Z., Novotná, M., and Hanusová, J. (2005). Body height, body mass index, waist-hip ratio, fluctuating asymmetry and second to fourth digit ratio in subjects with latent toxoplasmosis. Parasitology, 130(6), 621-628. DOI: 10.1017/s0031182005007316.
Lopes, F. M. R., Gonçalves, D. D.,Mitsuka-Breganó, R., Freire, R. L., and Navarro, I. T. (2007). Toxoplasma gondiiinfection in pregnancy. Brazilian Journal of Infectious Diseases, 11(5), 496-506. DOI: 10.1590/s1413-86702007000500011.
Als-Nielsen, B., Chen, W., Gluud, C., and Kjaergard, L. L. (2003). Association of funding and conclusions in randomized drug trials: a reflection of treatment effect or adverse events? Jama, 290(7), 921-928. https://doi.org/10.1001/jama.290.7.921.
Karagianni, E. E., Papazachos, C. B., Panagiotopoulos, D. G., Suhadolc, P., Vuan, A., and Panza, G. F. (2005). Shear velocity structure in the Aegean areaobtained by inversion of Rayleigh waves. Geophysical Journal International, 160(1):127-143.https://doi.org/10.1111/j.1365-246X.2005.02354.x
Jha, S. K., and Kannan, S. (2016). Serum prolactin in patients with liver disease in comparison with healthy adults: Apreliminary cross-sectional study.International Journal of Applied and Basic Medical Research, 6(1), 8-10. DOI:10.4103/2229-516X.173984
Hussein, R. D., and Mohammed, A. S.(2022). Relationship Between Toxoplasma gondii and Sex Hormone. EgyptianAcademic Journal of Biological Sciences. C, Physiology and Molecular Biology, 14(2), 229-236. https://doi.org/10.21608/eajbsc.2022.268229.
awaguchi, M., Takahashi, M., Hata, T., Kashima, Y., Usui, F., Morimoto, H., Izawa, A., Takahashi, Y., Masumoto, J., Koyama, J., Hongo, M., Noda, T.,
Nakayama, J., Sagara, J., Taniguchi, S., and Ikeda, U. (2011). Inflammasomeactivation of cardiac fibroblasts is essential for myocardial ischemia/reperfusion injury. Circulation, 123(6), 594-604. DOI:10.1161/CIRCULATIONAHA.110.982777
Shirbazou, S., Delpisheh, A., Mokhetari, R., and Tavakoli, G. (2013). Serologic detection of anti-Toxoplasma gondiiinfection in diabetic patients. Iranian Red Crescent Medical Journal, 15(8), 701-703. DOI: 10.5812/ircmj.5303.
Oz, H. S. (2014). Toxoplasmosis,pancreatitis, obesity and drug discovery. Pancreatic Disorders & Therapy, 4(2), 138 142.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4270089/pdf/nihms-644105.pdf
Hussien, J. H., Mohammed, A. A., an Aljorani, R. H. (2015). Study the effect of acute toxoplasmosis infection on some hormones and the phagocytic activity of neutrophils in pregnant and non-pregnant women before and after treatment.International Journal of Current Microbiology and Applied Sciences, 4(10), 459-465. https://www.ijcmas.com/vol-4-10/Jaber%20Hameed%20Hussien,%20et%20al.pdf
Wu, Y., You, S., Zang, H., Liu, H., Mao, Y., Mao, P., Zhu, B., Xu, J., Xie, G., Guo, J., Li, D., Xin, S., and Wan, Z. (2015). Usefulness of serum thyroid-stimulation hormone (TSH) as a prognostic indicator for acute-on-chronic liver failure. Annals of Hepatology, 14(2), 218 - 224. https://linkinghub.elsevier.com/retrieve/pii/S1665-2681(19)30784-7
Kharb, S., Garg, M. K., Puri, P., Brar, K. S., Pandit, A., and Srivastava, S. (2015). Assessment of thyroid and gonadal function in liver diseases. Indian journal of endocrinology and metabolism, 19(1), 89-94. DOI: 10.4103/2230-8210.131761
Kaňková, Š., Prochazkova, L., Flegr, J., Calda, P., Springer, D., and Potlukova, E.(2014). Effects of latent toxoplasmosis on autoimmune thyroid diseases in pregnancy. PLoS One , 9(10), e110878. doi:10.1371/journal.pone.0110878
Liu, X. C., He, G. D., Lo, K., Huang, Y. Q., and Feng, Y. Q. (2021). The triglycerideglucose index, an insulin resistance marker, was non-linear associated with all-cause and cardiovascular mortality in the general population. Frontiers in Cardiovascular Medi c ine , 7(10), 628-109. DOI:
3389/fcvm.2020.628109
Punekar, P., Sharma, A. K., and Jain, A. (2018). A study of thyroid dysfunction in cirrhosis of liver and correlation with severity of liver disease. Indian Journal of Endocrinology and Metabolism, 22(5), 645-651. doi: 10.4103/ijem.IJEM_25_18
Delitala, A. P., Fanciulli, G., Maioli, M., and Delitala, G. (2017). Subclinicalhypothyroidism, lipid metabolism andcardiovascular disease. European Journal of Internal Medicine, 38(10), 17-24. DOI: 10.1016/j.ejim.2016.12.015
El-Henawy, A. A. E. R., Abdel-Razik, A., Zakaria, S., Elhammady, D., Saudy, N., and Azab, M. S. (2015). Is toxoplasmosis a potential risk factor for liver cirrhosis? Asian Pacific Journal of Tropical Medicine, 8(10),784-791. DOI: 10.1016/j.apjtm.2015.09.00
Mani, R. S., Amin, M. A., Li, X., KalyanaSundaram, S., Veeneman, B. A., Wang, L., Ghosh, A., Aslam, A., Ramanand, S. G., Rabquer, B. J., Kimura, W., Tran, M., Cao, X. , Roychowdhury, S., Dhanasekaran, S. M., Palanisamy, N., Sadek, H. A., Kapur, P., Koch, A. E., and Chinnaiyan, A. M. (2016). Inflammationinduced oxidative stress mediates gene fusion formation in prostate cancer. Cell Reports, 17(10), 2620-2631. doi:
1016/j.celrep.2016.11.019
Gullu, S., Sav, H., and Kamel, N. (2005). Effects of levothyroxine treatment onbiochemical and hemostasis parameters in patients with hypothyroidism. European Journal of Endocrinology, 152(3), 355-361. DOI: 10.1530/eje.1.01857
Nafal, R. H., Al-Warid, H. S., and AlSultan, H. J. (2019). Seroprevalence of Toxoplasmosis in patients with chronic liver disease in Baghdad. Iraqi Journal of Science, 60(8), 1667 - 1672. https://doi.org/10.24996/ijs.2019.60.8.2
Carvalho, J. R., and Machado, M. V. (2018). New insights about albumin and liver disease. Annals of Hepatology, 17(4), 547-560.DOI: 10.5604/01.3001.0012.0916
Dooley, S. and Ten Dijke, P. (2012). TGF-β in progression of liver disease. Cell and Tissue Research, 347(1), 245-256. https://doi.org/10.1007/s00441-011-1246-y.
Zare-Bidaki, M., Assar, S., Hakimi, H., Abdollahi, S. H., Nosratabadi, R.,Kennedy, D., and Arababadi, M. K.(2016). TGF-β in Toxoplasmosis: Friend or foe? Cytokine, 86(9), 29-35.DOI:10.1016/j.cyto.2016.07.002.
Robert-Gangneux, F., and Dardé, M. L.(2012). Epidemiology of and diagnostic strategies for toxoplasmosis. Clinical Microbiology Reviews, 25(3), 264-296. DOI: 10.1128/CM
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