Combined effects of heavy metals and parasite infections on the haematological and biochemical profiles of wild rats from Awotan Municipal Dumpsite in Ibadan City
https://dx.doi.org/10.4314/njpar.v42i2.17
Keywords:
Helminths, Blood parasites, Heavy metals, Cortisol, Rat, Awotan Municipal DumpsiteAbstract
The combined effects of heavy metals and parasitic infections on wild rats from Awotan municipal dumpsite in Ibadan city southwest Nigeria were determined. A total of thirty rats were captured from the dumpsite and five rats from a control site using live traps. Rats were identified, weighed and body length measured to determine body condition index. Blood samples were collected from the rats using the cardiac puncture method. Rats were euthanized and dissected to extract various organs (kidney, liver and intestine). Intestinal helminths were extracted, identified and preserved. Blood parasites were identified using Giemsa staining technique of thick and thin films while intestinal helminths recovered were preserved and identified to species level. Organs, intestinal helminths and blood samples were digested and concentrated for heavy metals (Fe, Cu, Cr, Cd, and Pb) usingAtomicAbsorption Spectrophotometry (AAS). Cortisol level was determined using ELISAtechnique.All statistical significance was at probability value p < 0.05. Three species of rats were captured, Rattus rattus, Rattus novergicus and Cricetomys gambianus. The mean ± sd of the body condition index of the rats from dumpsite and control groups were 1.82±0.17 and 1.59± 0.38 respectively. There were significant differences in the mean values of eosinophils, platelets and white blood cell counts of rats from the two study sites. Overall parasite prevalence in dumpsite and control were 76.6% and 20% respectively. The overall prevalence of parasite species from dumpsite was in the order Hymenolepis diminuta (26.6%) >Monoliformis monoliformis (20%) >Plasmodium spp (20%) >Trypanosoma spp (10%). The mean concentration (mg/kg) of heavy metals in kidney, liver, intestine and blood of wild rats from both dumpsite and control decreased in the order Fe> Cr> Cu> Cd> Pb. Cortisol level was higher in infected rats from dumpsite (351±58.83ng/ml) than in uninfected rats from dumpsite (237.22± 44.46ng/ml), indicating exposure to immune stressors. A higher prevalence of parasitic infection, heavy metal pollution and the elevated cortisol concentrations in infected rats showed that the animals’ health and well-being are compromised.
References
Abila B, Kantola J. Existing Waste Management Processes and Practices in Nigeria. Int J Environ Ecol Geol Min Eng [Internet]. 2013;7(6):303–8.Available from: http://waset.org/publications/4713
Hoornweg D, Bhada-Tata P. A global review of solid waste management. Urban Dev Ser Knowl Pap [Internet]. 2012;1–116. A v a i l a b l e f r o m : https://openknowledge.worldbank.org/hand le/10986/17388
Adeyemo A and, Morenikeji O. Helminths and heavy metals in soils from a dumpsite in Ibadan city, Nigeria. J Prev Med Hyg. 2017;58(1):328–33.
International Solid Waste AssociationI S WA . G l o b a l i z a t i o n a n d Wa s t e Management. Iswa [Internet]. 2012;55. A v a i l a b l e f r o m : http://www.google.com/url?sa=t&rct=j&q= &esrc=s&source=web&cd=1&ved=0CCg QFjAA&url=http://www.iswa.org/index.ph p? eID=tx_iswatfg_download&fileUid=36 &ei=cTAZU67EF8WShgey7oC4Dg&usg= AFQjCNHFWggggcViVYGCyl6wHNRQ mnGtHQ&sig2=_-DffBNFZF7gcqX-U9- bCQ&bvm=bv.62578216
Ogunrinola IO, Adepegba EO. Health and Economic Implications of Waste Dumpsites in Cities: The Case of Lagos, Nigeria. Int J Econ Financ [Internet]. 2012 Mar 2 7 ; 4 ( 4 ) : 2 3 9 – 5 1 . Av a i l a b l e f r o m : http://www.ccsenet.org/journal/index.php/ij ef/article/view/15929
Angaye TCN, Abowei JFN. Review on the Environmental Impacts of Municipal Solid Waste in Nigeria: Challenges and Prospects. Greener J Environ Manag Public Saf [Internet]. 2017 Jun 30;6(2):018–33. A v a i l a b l e f r o m : h t t p : / / g j o u r n a l s . o rg / G J E M P S / g j e m p s - archive/vol-62-june-2017/angaye- andabowei.html
Angaye TCN. Journal of Medical Bioavailability of Some Heavy Metals Associated With Municipal Solid Waste Dumpsites in Yenagoa. 2018;4–8.
Rehman K, Fatima F, Waheed I, Akash MSH. Prevalence of exposure of heavy metals and their impact on health c o n s e q u e n c e s . J C e l l B i o c h e m . 2018;119(1):157–84.
Sures B, Siddall R, Taraschewski H. Parasites as Accumulation Indicators of. 1999;15(1):2–6.
F r a n k S N , S i n g e r C , S u r e s B . Metallothionein (MT) response after chronic palladium exposure in the zebra mussel, Dreissena polymorpha. Environ Res [Internet]. 2008 Nov;108(3):309–14. A v a i l a b l e f r o m : h t t p s : //linkinghub.elsevier.com/retrieve/pii/S001 3935108001643
Torres J, Eira C, Miquel J, Foronda P, Feliu C. Cadmium and lead concentrations in M o n i l i f o r m i s m o n i l i f o r m i s (Acanthocephala) and Rodentolepis microstoma (Cestoda), and in their definitive hosts, Rattus rattus and Mus domesticus in El Hierro (Canary Archipelago, Spain).Acta Parasitol [Internet]. 2011 Jan 1;56(3):320–4. A v a i l a b l e f r o m : h t t p : / / www.degruyter.com/view/j/ap.2011.56.issu e-3/s11686-011-0064-4/s11686-011- 0064- 4.xml
Sures B. Environmental parasitology: relevancy of parasites in monitoring environmental pollution. Trends Parasitol [Internet]. 2004 Apr;20(4):170–7. Available f r o m : h t t p s : / / linkinghub.elsevier.com/retrieve/pii/ S1471492204000297
Mavropoulos A, Mavropoulos A, Koukosia I, Tsakona M, Mavropoulou N, Rigas N, et al. Waste Atlas The World’s 50 Bigest Dumpsites 2014 Report [Internet]. Waste Atlas. 2014. p. 122. Available from: h t t p : / / w w w . d - waste.com/Atlasreport2014/Waste-Atlasreport-2014-webEdition.pdf
Mohd-Zain SN, Alias SN, Sahimin N, Edah M. Epidemiology of blood parasitic infections in the urban rat population in peninsular Malaysia Epidemiology of blood parasitic infections in the urban rat population in peninsular Malaysia. Trop Biomed. 2014;31(2):230–40.
Herbreteau V., Jittapalapong S., Rerkamnuaychoke W., Chaval Y. CJ-F and MS. Protocols for field and laboratory rodent studies. Retrieved from CERoPath project: [Internet]. 2011. 1-200 p. Available f r o m : http://www.ceropath.org/FichiersComplem entaires/Herbreteau_Rodents_protocols_20 11.pdf
Siti Shafiyyah, C.O., Jamaiah, I., Rohela, M., Lau, Y.L. and Siti Aminah F. Prevalence of intestinal and blood parasites among wild rats in Kuala Lumpur, Malaysia. Trop Biomed. 2012;29(4):544–50.
Rai SK, Uga S, Kataoka N, Matsumura T. Atlas of Medical Parasitology. Am J Public Heal Nations Heal. 1996;42(2):203.
Sepulveda MS, Kinsella JM. Helminth Collection and Identification from Wildlife 1. Animal Necropsy and Screening of Major Organs for Helminth Collection. 2013;(December):1–5.
Teimoori S, Yaraghi AS, Makki MS. Heavy Metal Bioabsorption Capacity of Intestinal H e l m i n t h s i n U r b a n R a t s . 2014;43(3):310–5.
Mizukawa H, Ishizuka M, Ikenaka Y, Akoto O, Baidoo E, Nakayama S, et al. Heavy metals and metalloid accumulation in livers and kidneys of wild rats around gold-mining communities in. J Environ Chem Ecotoxicol. 2016;8(7):58–68.
Krebbs, Charles J; Singleton GD. Indices of Condition for Small Mammals. Australia Journal of Zoology. 1993; 41(317- 23).
Gurler AT, Beyhan YE, Bolukbas CS, Acici M, Umur S. Gastro-intestinal helminths of wild rats (brown rat Rattus norvegicus Berkenhout1769)in Samsun, Turkey. Vet Fakültesi Derg. 2016;58(June):48–50.
Paul B, Kyari F, Gadzama M, Zango M, Ejeh E, Ndahi J, et al. Gastrointestinal helminths and external parasites of domestic rats trapped from residential areas within Maiduguri Municipality, Nigeria. Sokoto J Vet Sci [Internet]. 2016;14(1):10. Available f r o m : http://www.ajol.info/index.php/sokjvs/articl e/view/135205
Isaac C, Igbinosa Igho B, Ohiolei AJ, Osimen EC. Endoparasite of small mammals in Edo State, Nigeria: Public Health Implications. Korean J Parasitol [Internet]. 2018;56(1):93–100. Available f r o m : https://doi.org/10.3347/kjp.2018.56.1.93
Ayinmode, A.B; Ndudim, F.M. and Obebe OO. Prevalence of Gastrointestinal Parasites of Rodents in Ibadan, Nigeria. 2012;36(2):1158–64.
Tongo I, Ewere EE, Awharitoma AO, Akenbor SA, Omoriyekemen GO. Tissue and parasite accumulation of heavy metals in the giant rat (Cricetomys gambianus) as bioindicators of heavy metal pollution. J Appl Sci Environ Manag [Internet]. 2018 Nov 30;22(10):1667. Available from: https://www.ajol.info/index.php/jasem/artic
le/view/180271
Alimba CG, Bakare AA. Municipal landfill leachates induced alterations in haematological indices and erythrocyte morphology of rats. Int J Chem Environ Eng Syst. 2012;3(3):84–100.
Balogun T, Morenikeji O, Emikpe B, Oyebanji V. Clinicopathological features observed in rats exposed to leachates from a municipal dump site in Nigeria Clinicopathological features observed in rats exposed to leachates from a municipal dump site in Nigeria. Zool Ecol [Internet]. 2017;(November):1–6. Available from: http://doi.org/10.1080/21658005.2017.140 0741
Sures B, Scheef G, Klar B, Kloas W, Taraschewski H. Interaction between cadmium exposure and infection with the i n t e s t i n a l p a r a s i t e M o n i l i f o r m i s moniliformis (Acanthocephala) on the stress hormone levels in rats. Environ Pollut [Internet]. 2002 Oct;119(3):333–40. A v a i l a b l e f r o m : https://linkinghub.elsevier.com/retrieve/pii/ S0269749101003402
Durojaye AS, Olufemi MA, Rasheed YO, Ibukun AA. Bioaccumulation of heavy metals in cane rat (Thryonomys swinderianus) in Ogun State, Nigeria. J Toxicol Environ Heal Sci [Internet]. 2014 Oct 31;6(8):154–60. Available from: http://academicjournals.org/journal/JTEHS/ article-abstract/9E0A08347674
Jankovská I, Langrová I, Bejcek V, Miholová D, Vadlejch J, Petrtyl M. Heavy metal accumulation in small terrestrial rodents infected by cestodes or nematodes. Parasite [Internet]. 2008;15(4):581–8. Available from: http://www.parasitejournal.org/10.1051/parasite/2008154581
Hassan A, Moharram S, Helaly H El. Role of Parasitic Helminths in Bioremediating Some Heavy Metal Accumulation in the Tissues of Lethrinus mahsena. Turkish J Fish Aquat Sci. 2018;443:435–43.
Barus V, Tenora F, Sumbera R. Relative concentrations of four heavy metals in the p a r a s i t e s P ro t o s p i r u r a m u r i c o l a ( N e m a t o d a ) a n d I n e r m i c a p s i f e r arvicanthidis (Cestoda) in their definitive host silvery mole-rat. Helmitnhologia. 2016;40(December 2003):227–32.
Kovalchuk LA, Mikshevich N V, Chernaya L V. Accumulation of Heavy Metals by Small Mammals the Background and Polluted Territories of the Urals. Vestn Zool. 2017;51(4):325–34.
Jankovská I, Langrova I, Bejcek V, Miholova D, Vadlejch J, Petrtyl M. Heavy metal accumulation in small terrestrial rodents infected by cestodes or nematodes. Parasite J. 2008;15(-):581–8.
Sures B, Dezfuli BS, Krug HF. The intestinal p a r a s i t e P o m p h o rh y n c h u s l a e v i s (Acanthocephala) interferes with the uptake and accumulation of lead (210Pb) in its fish host chub (Leuciscus cephalus). Int J Parasitol. 2003;33(14):1617–22.
Sures B, Nachev M, Selbach C, Marcogliese DJ. Parasite responses to pollution: what we know and where we go in “Environmental Parasitology.” Parasit Vectors [Internet]. 2017 Dec 6;10(1):65. Available from: http://parasitesandvectors.biomedcentral.co m/articles/10.1186/s13071-017-2001-3
Tripodi MA, Hancke D, Suarez OV. Analysis of lead pollution levels within an urban ecosystem using the cestode Hymenolepis diminuta and its rat hosts as bioindicators. J Helminthol [Internet]. 2018 Sep 11 ; 9 2 ( 5 ) : 5 4 4 – 8 . Av a i l a b l e f r o m : https://www.cambridge.org/core/product/id entifier/S0022149X17000876/type/journal _article
Mustafa A, Piasecki W. Factors affecting the susceptibility of sea lice infection in Atlantic s a l m o n . Wi a d o m o ś c i P a r a z y t o l . 2005;51(4):281–5.
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