Molecular Detection of Trypanosoma species in Cattle Slaughtered at Zango and Yantukwane Abattoirs in Zaria, Kaduna State, Nigeria
https://doi.org/10.4314/njpar.v41i2.7
Keywords:
infection, breed, Sokoto Gudali, White Fulani, TrypanosomosisAbstract
African animal trypanosomoses constitute the most important vector-borne cattle diseases in Sub-Saharan Africa. A total of 170 blood samples were collected from cattle in Zango abattoir and Yantukwane abattoir in Zaria, Kaduna State. Blood Samples collected were examined for the presence of trypanosomes using the buffy coat technique and Giemsa thin blood smears which shows a single 1(0.58%; 1/170) infection using microscope. Positive and negative samples were subjected to further analysis using nested PCR reaction with nested primers for detection of trypanosomes, and 9 of the samples showed positive bands after gel electrophoresis at 998bp (lane 1-9) indicating the presence of Trypanosoma theileri. Percentage, Chisquare and Odds ratio were used to analyse the data.Atotal of 9(5.29%; 9/170) animals were found infected with the parasite out of which 4(4.65%; 4/85) were found in Zango abattoir, while 5 (5.95%) were found in Yantukwane abattoir. The results were not statistically (P>0.05) location dependent. The infection in male (6.45%) cattle was higher than the female (4.63%) with no infection in young animals. No infection was observed in Sokoto Gudali breed, while White Fulani breed had 5.77%. From this study, it is clear that trypanosomosis is still a major obstacle to livestock production in Zaria and its Environs and the incidence is majorly in adult cattle. In addition, nested PCR reaction showed more sensitivity than microscopy. Hence, there is need for proper diagnosis and treatment of animals to promote more beef and milk production for human consumption. Further study is necessary to determine the pathogenicity of T. theileri in other domestic animals.
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