Ameliorative effect of Quercetin and Omega-3 Fatty Acids on Haematological and Liver function Impairment induced by Mosquito Coil smoke in male Wistar Rats

Authors

  • E. O. Aribo Department of Physiology, University of Calabar, Calabar, Nigeria.
  • S. U. Uquetan Department of Physiology, University of Uyo, Uyo, Nigeria
  • A. E. Phillip Department of Physiology, University of Calabar, Calabar, Nigeria.
  • A. O. Uduak Department of Physiology, University of Uyo, Uyo, Nigeria

DOI:

https://doi.org/10.4314/njpar.v45i1.14

Keywords:

Omega-3, Quercetin, hepatic impairment, haematological, Mosquito coil

Abstract

The use of mosquito coils is one method adopted to control malaria Inhalation of the coil smoke causes adverse effects, including hepatic and haematological toxicities associated with oxidative stress. Therefore, this study investigated the effects of phytochemical antioxidants (Quercetin and Omega-3) on mosquito coil smoke-induced haematological and hepatic impairments. Twenty male Wistar rats were divided into 4 groups viz control, mosquito coil-only (MC), mosquito coil+Quercetin (MC+Q) and mosquito coil+Omega-3 (MC+OM3) groups of five rats each. Quercetin and Omega-3 were administered daily at 20mg/kg and 600mg/kg respectively. The duration of daily exposure and treatment was 42 days after which blood samples were collected via cardiac puncture for determination of relevant haematological and hepatic parameters. The RBC count was significantly reduced in the MC group compared with the control (P<0.05) but was higher in the MC+Quercetin group than in the MC group. Haemoglobin was decreased in the MC and MC+Omega-3 groups compared with the control (P<0.05). White blood cell count was higher in the MC group than in the control group (P<0.05) but lower in
the MC+OM3 and MC+Q groups than in the MC group (P<0.05). The lymphocyte count was higher in the MC group than in the control group (P<0.05). Serum aspartate transaminase and alanine transaminase were significantly increased (P<0.05) in the MC group compared with the control group but were reduced (P<0.05) in the MC+OM3 and MC+Q groups compared with the MC group. Serum alkaline phosphatase was significantly higher (P<0.05) in the MC group than in control (P<0.05) but significantly lower (P<0.05) in the MC+OM3 group than in the MC group. Serum total bilirubin was significantly elevated in the MC group compared to that in the control group (P<0.05). The serum concentration of direct bilirubin was significantly higher (P<0.05) in the MC group than in the control group. We conclude that Omega-3 and Quercetin ameliorate haematological and hepatic function impairments in Wistar rats exposed to mosquito coil smoke.

         Views | Download: 72 / 0

References

Centre for Disease Control and Prevention (2023). Malaria Impact Worldwide. https://www.cdc.gov.malaria. Retrieved 23 September 2023

World Health Organization (2022). World Malaria Report, 2016. Geneva. World Health Organization. https://www.who.int/malaria/report.

Retrieved 26 October 2023

Sabina, K. (2017). Prevalence and epidemiology of malaria in Nigeria: a review. International Journal of Research in Pharmacy and Biosciences 4(8):10–12.https://www.reserachgate.net.

Garba, S., Adelaiye, A. B., and Mshelia, L. Y. (2007). Histopathological andBiochemical changes in the rat’s kidney following exposure to pyrethroid-based mosquito coil. Journal of Applied Sciences Research, 3(12): 1788 – 1793. https://www.researchgate.net

Abdulah, M. A., Ataur, M.R., Hoque, K. M., Ferdousi, Z., Mohammad, M. N. and Abu, R. M. (2017). Biochemical and Histological Alterations induced by the smoke of allethrin-based mosquito coil on mice model. BMC Clinical Pathology, 17(19):1–8. Doi:10:1186/s12907-017-

-9.

El-Demerdash, F. M. (2011). Oxidative stress and hepatoxicity induced by synthetic pyrethroids organophosphate insecticides mixture in rats. Journal of Environmental Sciences and Health, 29:145–158.doi:10.1080/10590501.2011.5776679

Daniel, S., Frances, S. P. and Debboun, M. (2009). Prevention of bug bites stings and disease. New York. Oxford University Press. https://www.amazon.com. Retrieved 19 September, 2023

Chen, S. C., Wong, R. H., Shiu, L. J., Chiou, M. C. and Lee, H. (2008). Exposure to mosquito coil smoke may be a risk factor for lung cancer in Taiwan. Journal of Epidemiology, 18(1):19–25. doi:10.2188/jea.18.19.

Koo, L. C. and Ho, J. H. C. (1994). Mosquito coil smoke and respiratory health among Hong Kong Chinese. Epidemiological studies. Indoor Environment, 3: 304 – 310. https://doi.org/10.1177/1420326X9400300510.

Idowu, E. T., Aimufua, O. J., Ejovwoke, Y. O., Akinsanya, B., and Otubanjo, O. A. (2013). Toxicological effects of prolonged and intense use of mosquito coil emission in rats and its implications on malaria control. Revistade Biologia Tropical, 61(3)1463–1473.

https://pubmed.ncbi.nlm.nih.gov/24027936/

Okoh, H. I., Odoh, I. M., Ahmed, J., Oluwafemi, F. and Morikwe, U. C. (2023). Effects of mosquito coil inhalation on the ha ema tologi c a l, biochemi c a l and histopathological parameters of wistar rat. Nigerian Journal of Parasitology,44(1):201–208. doi:10.4314/njpar.v44i1.20

Madhubabu, G. and Yenugu, S. (2012). Effect of continuous inhalation of alletrinbased mosquito coil smoke in male reproductive tract of rats. Inhalation Toxicology, 24(3):143-152. https://doi.org/10.3109/08958378.2011.649189

Boots, A. W. (2006). Health Effects of Quercetin: from Mechanism to Nutraceutical. PhD Thesis of the University of Maastricht , Netherlands.

https://pubmed.ncbi.nlm.nih.gov.doi:10.1016/ejphar.2008.03.008.

Bast, A. and Haenen, G. R. (2002). The toxicity of antioxidants and their metabolites.Environmental Pharmacology, 11:251-258.

doi:10.1016./s1382.6689(01)00118-1.

Haenen, G. R. and Bast, A. (2002). The use of vitamins in self medication. Therapie 57:119-222.https://pubmed.ncbi.nlm.nih.gov/12185958

/

van Acker, S. A., Tromp, M. N., Haenen, G.R., van der Vijgh, W. J. and Bast, A(1995). Flavonoids as scavengers of nitric oxide radicals. Biochemical and Biophysical Research Communication, 241(3):755-759. https://doi.org/10.1006/bbrc.1995.2350

Heijnen, G. C., Haenen, G. R., van Acker, F. A., van der Vijgh, W. J. and Bast, A(2001). Flavonoids as peroxinitrite scavengers: the role of the hydroxyl groups. Toxicology in vitro: An International Journal Published in association with BIBRA, 15(1):3–6. https://doi.org/10.1016/s0887-2333(00)00053-9

Hajianfar, H., Paknahad, Z. and Bahonar, A. (2013). The effect of Omega-3 supplementation on antioxidant capacity in patients with Type 2 diabetes. International Journal of Preventive Medicine 4(Suppl 2): S234-S238 https://www.ncbi.nlm.nih.gov.

Kofue, T. C., Djote, W. N. B., Marilyne, M., Pieme, A. C., Kansci, G. and Foko, E. (2019). Anti-sickling and antioxidant properties of Omega-3 fatty acids EPA/DHA. Nutrition and Food Sciences International Journal, 9(1):555752. https://www.juniperpublishers.com.

Aribo, E. O., Sunday, V. E., and Udokang, N. E. (2023). Effects of Omega-3 and Selenium on haemotoxicity induced by HAART in Wistar Rats. Western Journal of Medical and Biomedical Sciences, 4(1-2):47-55). https:/doi.org/10.5281/zendo.8143810

Siddique, N., Nazir, S. and Jabeen, R.(2016). Mosquito coil inhalation effects on interstitium of kidney of Albino rats.Pakistan Journal of Medical and Health Sciences, 9(2):627-675. https://www.pjmhsonline.com/2015/apr_june/pdf/672%20%20%20Mosquito%20Coil%20Smoke%20Inhalation%20Effects%20on%20Interstitium%20of%20Kidney%20of%20Albino%20Rats.pdf

Okine, L. K. N., Nyarco, A. K., Armar, G.E., Awumbila, B., Owusi, K., Ser-soafia, S. and Ofosuhe, M. (2004). Adverse effect of mosquito coil smoke on lung, liver and certain drug-metabolising enzymes in male Wistar rat. Ghana Medical Journal,38(3):89-95 doi:10.4314/gmj.v38i3.36001.

World Population Review (2023). Average bedtime by Country. https://www.worldpopulationreview.com.Retrieved 17 November 2023

Darcin, P. and Balanli, A. (2020).Examining the effect of space on indoor air pollution exposure. ICONARCH IV, International Congress of Architecture and Planning Space and Process in Architecture and Planning, Konya, 13-15 October 2020, pp: 403-412 (ISBN: 978-625-7327-00-8).

https://www.researchgate.net

Li, C. and Managi, S. (2022). Spatial Variability of the relationship between air pollution and well being. Sustainable Cities and Societies, 76 2022 103447 ISSN 2210-6707,https://doi.org/10.1016/j.scs.2021.103447.

Abubakar, M and Hassan, L.(2006). Some Toxicological effects of some mosquito coil brands in Experimental Rats. The Internet Journal of Toxicology, 4:1 https//:spub.com doi:10.5580/5d8

Reitman, S. and Frankel, S. (1957). A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. American Journal of Clinical Pathology, 28(1):56-63. https://doi.org/10.1093/ajcp/28.1.56.

Schlebusch, H., Rick, W., Lang, H., and Knedel, M. (1974). Standards in the activities of clinically important enzymes. Deutsche medizinische Wochenschrift,99(15):765–766. https://doi.org/10.1055/s0028-1107840

Walters, M. I. and Genarde, H. W. (1970). An ultramicro method for determination of conjugated and total bilirubin serum or plasma. MicroChemical Journal, 15(2): 231-24. doi:10.1016/0026.265X(70)90045-7.

Abo-El Sooud, K., Abd-El Hakim, Y. M., Hashim, M. M. M., El-metwally, A. E., Hassan, B. A. and El-Nour, H. H. M. (2023). Restorative effect of garlic acid against sub-chronic hepatic toxicity of coexposure to zinc oxide nanoparticles and arsenic trioxide in male rats. Heliyon 9(6)e17326.

https://doi.org/10.1016/j.heliyon.2023.e17326

Ibiam, U. A. and Ugwu, O. (2013). Rambo mosquito coil smoke induced liver toxicity and the protective effect of G. Latifolium leaf extract. An Abstract of a paper presented at 2nd International Summit on Toxicology in Las Vega. https://www.longdom.com.Retrieved 17 September, 2023

Haase, V. H. (2013).Regulation oferythropoiesis by hypoxia-inducible factors. Blood Reviews, 27(1):41-53. https://doi.org/10.1016/j.blre.2012.12.003.

Remigante, A., Spinelli, S., Straface, E., Gambardela, L., Caruso, D., Falliti, G., Dossena, S., Marino, A. and Morabito, R. (2022). Antioxidant Activity of Quercetin in H2O2-induced oxidative stress model in Red Blood Cells: Functional role of Band 3 protein. International Journal of Molecular Sciences. 23(19):10:991 https//:doi.org./10.3390/ijms231910991.

Velguth, K. E., Payton, M. E, and Hoover, J. E. (2010). Relationship of haemoglobin concentration to packed cell volume.Journal of Avian Medicine and Surgery,24(2):115-121. https://doi.org/10.1647/2008-042.1.

Kumar, V., Abbas, A. K., Fausto, N. and Mitchell, R. S. (2007). Robbins Basic Pathology. Philadelphia. Saunders Elsevier.

Guyton, A. C. and Hall, J. E. (2011). Textbook of Medical Physiology.Philadelphia. Saunders Elsevier.

Lidbury, J. A. and Steiner, J. M. (2013). Diagnostic evaluation of the liver. InWashabau R.J, Day M.J (eds): Canine and Feline Gastroenterology, St Louis, MO, Saunders Elsevier.

Gowda, S., Prakash, B. D., Sonal, V., Shruthi, K., Vinayak, H., & Avinash, M.(2009). Thyroid function tests: a review. European Review for Medical and Pharmacological Sciences, 13(5), 341–349. https://doi.org/10.11604/pamj.2009.3.17.125.

Pizzino, G., Irrera, N., Cuccinota, M., Pallio, G., Arcoraci, V., Squadrito, F., Allavila, D. and Bitto, A. (2017). Oxidative Stress: Harms and Benefits for Human Health. Oxidative Medicine and Cellular Longevity. 2017:8416763. https://doi.org/10.1155/2017/8416763.

Published

2024-04-02

How to Cite

Aribo, E. O., Uquetan, S. U., Phillip, A. E., & Uduak, A. O. (2024). Ameliorative effect of Quercetin and Omega-3 Fatty Acids on Haematological and Liver function Impairment induced by Mosquito Coil smoke in male Wistar Rats. Nigerian Journal of Parasitology, 45(1), 126–134. https://doi.org/10.4314/njpar.v45i1.14

Similar Articles

1 2 3 > >> 

You may also start an advanced similarity search for this article.