Molecular Characterisation and Phylogenetic Analysis of <i>Toxoplasma gondii</i> Isolates from HIV-positive Individuals in Calabar, Nigeria
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Abstract
Toxoplasma gondii is a very successful zoonotic parasite that can infect virtually all warm-blooded animals, including humans. Marked biological differences exist among T. gondii strains in their pathogenicity and geographical distribution, but the relatedness of the prevalent strains in the study area to existing global strains has not been investigated. This study aimed to investigate the molecular prevalence and phylogenetic analysis of T. gondii among HIV-positive individuals in Calabar, Nigeria, to provide information on the relatedness of T. gondii strains in Nigeria to existing global strains in GenBank. In a cross-sectional study, blood samples were randomly collected from 50 confirmed HIV/Toxoplasma gondii seropositive individuals (aged 10-65 years and amplified by Polymerase Chain Reaction (PCR) using the Tox G1 gene. Sequencing of the selected amplicons was performed using the Sanga sequencing method. The molecular prevalence of T. gondii, as determined by PCR, was 70%. Neither age nor sex affected PCR amplification of the Tox G1 gene (P >0.05). Phylogenetic analysis of the sequenced gene using the Basic Local Alignment Tool (BLAST) revealed that 60% of the T. gondii strains (T1–T10) detected in the study area were similar to those in Thailand, 30% (T6, T7, and T8) and Iran, 30% (T1, T2, T3), while 20% (T9, T10) were related to those detected in Norway. This study revealed a high molecular prevalence (70%) of Toxoplasma gondii among HIV-positive individuals in Calabar, Nigeria and demonstrated the genetic relatedness of local strains to those from Thailand, Iran, and Norway, underscoring the importance of PCR-based diagnosis and molecular surveillance in immunocompromised populations.
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References
Furtado, J. M., Smith, J. R., Belfort, R., Gattey, D. & Winthrop, K. L. (2011). Toxoplasmosis: A Global Threat. Journal of Global Infectious Diseases, 3 (3), 281-284. DOI:10.4103/0974-777X.83536
World Health Organisation (WHO) 2013. www.who.int/entity/tb/publications/2009/tbfactsheet_2009update_one_page.pdf, accessed July 2013.
Bahia-Oliveira, L., Gomez-Marin, J. and Shapiro, K. (2017). Toxoplasma gondii. In: J.B. Rose and B. JiménezCisneros (eds), Water and Sanitation for the 21st Century: Health and Microbiological Aspects of Excreta and Wastewater Management (Global Water Pathogen Project). (R. Fayer and W. Jakubowski (eds), Part 3: Specific Excreted Pathogens: Environmental and Epidemiology Aspects - Section 3: Protists), Michigan State University, E. Lansing, MI, UNESCO. https://doi.org/10.14321/waterpathogens.37
Sudan, V., Jaiswal, A.K. & Shanker, D.(2013). Recent trends in the diagnosis of Toxoplasmosis. Clinical Reviews and Opinions, 5(2), 11-17.
Nissapatorn, V. (2011). Toxoplasmosis in HIV/AIDS Patients- A Living Legacy, Microbes, Viruses and Parasites in AIDS Process, Prof. VladimÃr Zajac (Ed.), ISBN: 978-953-307-601-0. https://doi.org/10.5772/19580
Gyang, V. P., Akinwale, O. P., Lee, Y. L., Chuang, T. W., Orok, A., Ajibaye, O., Liao, C. W., Cheng, P. C., Chou, C. M., Huang, Y. C., Fan, K. H., & Fan, C. K. (2015). Toxoplasma gondii infection: Seroprevalence and associated risk factors among primary schoolchildren in Lagos City, Southern Nigeria. Revista da Sociedade Brasileira de Medicina Tropical, 48(1), 56-63. https://doi.org/10.1590/0037-8682-0310-2014
Lin, D. S. & Bowman, D. D. (1992). Toxoplasma gondii: an AIDS enhancing cofactor. Medical Hypothesis, 39(2), 140-142. https://doi.org/10.1016/0306-9877(92)90174-B
Nissapatorn, V., Lee, C., Quek, K. F., Leong, C. L., Mahmud, R., & Abdullah, K. A. (2004). Toxoplasmosis in HIV/AIDS patients: a current situation. Japanese Journal of Infectious Diseases, 57(4), 160–165. DOI: 10.7883/yoken.JJID.2004.160.
Skariah, S., McIntyre, M. K., & Mordue, D. G. (2010). Toxoplasma gondii: determinants of tachyzoite to bradyzoite conversion. Parasitology Research, 107(2), 253–260. https://doi.org/10.1007/s00436-010-1899-6
Dubey, J. P. (2010). General Biology. Toxoplasmosis of Animals and Humans. (Dubey, JP, ed.). Boca Raton, FL, CRC Press.
Weiss, L.M. & Kim, K. (2011). Toxoplasma gondii: The Model Apicomplexan. Perspectives and Methods. Elsevier. Academic Press. https://books.google.com.ng/books?id=yTUkJEphM_IC, ISBN: 9780080475011
Bastien, P. (2002). Molecular diagnosis of toxoplasmosis. Transactions of the Royal Society of Tropical Medicine and Hygiene, 96 Suppl 1, S205–S215. https://doi.org/10.1016/s0035-9203(02)90078-7
Ivovic, V., Vujanic, M., Zivkovic, T., Klun, I. & Djurkovic-Djakovic, O. (2012). Molecular Detection and Genotyping of Toxoplasma gondiifrom Clinical Samples, INTECH 5, 104-120. https://doi.org/10.5772/50830
O’Driscoll, J. C. & Holliman, R. E. (1991). Toxoplasmosis and bone marrow transplantation. Review of Medical Microbiology, 2, 215–222. cabidigitallibrary.org
Liu, Q., Wang, Z. D., Huang, S. Y., & Zhu, X. Q.(2015). Diagnosis of toxoplasmosis and typing of Toxoplasma gondii. Parasites & vectors, 8, 292. https://doi.org/10.1186/s13071-015-0902-6
Ottong, J. G., Ering, S. O. and Akpan, F. U. (2010). The population situation in Cross River State, Nigeria and its implications for socio-economic development: Observations from the 1991 & 2006 censuses. Journal of Emerging Trends in Educational Research and Policy Studies, 1(1):36-42. www.jeteraps.scholarlinkresearch.org
Saito, N. & Nei, M. (1987). The Neighbour-joining method: A new approach for reconstructing phylogenetic trees. Molecular Biology and Evolution, 4, 406-425. https://doi.org/10.1093/oxfordjournals.molbev.a040454
Felsenstein J. (1985). Confidence Limits On Phylogenies: An Approach Using The Bootstrap. Evolution; International Journal of Organic Evolution, 39(4), 783–791. https://doi.org/10.1111/j.1558-5646.1985.tb00420.xFelsenstein, J. (1985). Confidence limits on phylogenies. An approach using the bootstrap. Evolution, 39, 783-791.
Jukes, T. H. and Cantor, C. R. (1969). Evolution of Protein Molecules. In: Munro, H. N., Ed., Mammalian Protein Metabolism, Academic Press, New York, 21-132. http://dx.doi.org/10.1016/B978-1-4832-3211-9.50009-7
Gashout A., Amro A., Erhuma, M., Al-Dwibe H., Elmaihub E., Babba H., Nattah N. & Abudher A. (2016). Molecular diagnosis of Toxoplasma gondii infection in Libya. BMC Infectious Diseases, 16(1),157. DOI: 10.1186/s12879-016-1491-5
Bin Dajem, S. M., & Almushait, M. A. (2012). Detection of Toxoplasma gondii DNA by PCR in blood samples collected from pregnant Saudi women from the Aseer region, Saudi Arabia. Annals of Saudi Medicine, 32(5), 507–512. https://doi.org/10.5144/0256-4947.2012.14.7.1200
Burrells, A., Opsteegh, M., Pollock, K. G., Alexander, C. L., Chatterton, J., Evans, R., Walker, R., McKenzie, C. A., Hill, D., Innes, E. A., & Katzer, F. (2016). The prevalence and genotypic analysis of Toxoplasma gondii from individuals in Scotland, 2006-2012. Parasites & vectors, 9(1), 324. https://doi.org/10.1186/s13071-016-1610-6
Ibrahim, H. M., Mohamed, A. H., El-Sharaawy, A. A. & El-Shqanqery, H. E. (2017). Molecular and serological prevalence of Toxoplasma gondii in pregnant women and sheep in Egypt. Asian Pacific Journal of Tropical Medicine, 10(10): 996-1001. DOI: 10.1016/j.apjtm.2017.09.012.
Bahreh, M., Hajimohammadi, B. & Eslami, G. (2021). Toxoplasma gondii in Sheep and Goats from Central Iran. BMC Research Notes, 14, 46. https://doi.org/10.1186/s13104-021-05465-3
Anvari, D., Saadati, D., Nabavi, R. & Alipour Eskandani, M. (2018). Epidemiology and Molecular Prevalence of Toxoplasma gondii in Cattle slaughtered in Zahedan and Zabol Districts, South East Iran. Iranian Journal of Parasitology, 1: 13(1), 114-119.
Gómez-Marín, J.E., De-la-Torre, A., Angel-Muller, E., Rubio, J., Arenas, J. & Osorio, E. et al. (2011). First Colombian multicentric newborn screening for Congenital Toxoplasmosis. PLoS Neglected Tropical Diseases, 5(5): e1195. doi: 10.1371/journal.pntd.0001195.
Buddhirongawatr, R., Chaichoun, K., Tungsudjai, S., Udonsom, R., Thompson, A., Mahittikorn, O., Thompson, A., Dekumyoy, P. (2016). Seroprevalence and Phylogenetic Analysis of Toxoplasma gondii from Domestic Cats, Captive Wild Felids, Free-range Wild Felids and Rats in Certain Regions of Thailand. Thai Journal of Veterinary Medicine, 46(2), 209-218. DOI:10.56808/2985-1130.2728
Shin, H. G., Lee, S. E., Hong, S. H., Kim, S. M., Choi, Y. K., Park, H. J. et al. (2013). Prevalence of Toxoplasma gondii infection in rabbits of Korea by serological tests and nested polymerase chain reaction. Journal of Veterinary Medicine and Science, 75(12), 1609-1613. DOI: 10.1292/jvms.13-0360
Shapiro, K., VanWormer, E., Aguilar, B., & Conrad, P. A. (2015). Surveillance for Toxoplasma gondii in California mussels (Mytilus californianus) reveals transmission of atypical genotypes from land to sea. Environmental Microbiology, 17(11), 4177–4188. https://doi.org/10.1111/1462-2920.12685